in-vitro vs. in-vivo translation
wluo at UNIXG.UBC.CA
Mon Aug 15 16:21:01 EST 1994
I have two questions regarding detection of a protein:
1. Has anyone worked on a protein without Met? I have used 35S-Cys to
label the protein and it worked well by in vitro rabbit reticulocyte but
not in transfected cells.
2. If a protein is expressed by in vitro system, should it be
expressed in cells in general?
Any comments and suggestions will be appreciated.
More information about the Methods