Does pUC18 have a T7 promoter?

Mark Farinha mfarinha at gpu.srv.ualberta.ca
Mon Aug 15 15:08:29 EST 1994


In article <aquilla.1127368350C at sadye.emba.uvm.edu>(Tracy Aquilla) says:
>
>I have a map of pUC 18 which shows T7/T3 promoters downstream from the
>polylinker. Does anyone know if this is correct? 

the pUC series does NOT have T7/T3/SP6 promoters but many of the
commercial derivatives do: e.g. pGEM, Bluescript,etc. Many of these 
also have a filamentous phage origin of replication for single-strand
DNA production which is also absent in pUC. 



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