SDS removal

[Paul Riggs]

In article <CuB7Ds.5pF at liverpool.ac.uk>, agmclen at liv.ac.uk wrote:

> I am sure this must be an FAQ, but does anyone have an opinion on the best way
> to remove unbound and bound SDS from proteins recovered from a preparative SDS
> polyacrylamide gel that is mild enough so that significant renaturation and
> recovery of enzyme activity can ultimately be achieved?
> Thanks 

Check out Hager & Burgess (1980) "Elution of proteins from SDS-PAGE
Gels...", Anl. Biochem 109, 76-86. A few people here have used this method
successfully.
______
Paul Riggs
New England Biolabs
riggs at neb.com