Coomassie Blue G-250 staining

Dietmar Tietz Dietmar.Tietz at agrar.uni-giessen.de
Tue Aug 16 10:04:40 EST 1994


On 15 Aug 1994 Unknown at dl.ac.uk wrote:

> Does anyone have the information about using Coomassie Blue G-250 to 
> stain protein gels?
> 
> Please send your response to lin at mbimail.umd.edu
> 
> Thanks.
> 
> Lin 
> 
> 
> 
Lin,

You may find an overview in:

Chrambach, A.: The Practice of Quantitative
Gel Electrophoresis, VCH Weinheim 1985

Tietz, D.: J Chromatogr. 418, 305-344 (1987)

Detailed procedures are given in:

Diezel, W., G. Kopperschlaeger, G., and Hoffmann, E.: Anal.
Biochem., 48, 617-620 (1972)
 
Neuhoff, V., Stamm, R., and Eibl, H.: Clear background 
and highly sensitive protein staining with Coomassie Blue 
dyes in polyacrylamide gels: a systematic analysis. 
Electrophoresis 6, 427-448 (1985)
 
Good luck!

Dietmar Tietz

 
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