Coomassie Blue G-250 staining

Marion Freistadt mfreis at lsumc.edu
Tue Aug 16 12:50:22 EST 1994


>> Does anyone have the information about using Coomassie Blue G-250 to
>> stain protein gels?
>>
>> Please send your response to lin at mbimail.umd.edu
>>
>> Thanks.
>You may find an overview in:
>
>Chrambach, A.: The Practice of Quantitative
>Gel Electrophoresis, VCH Weinheim 1985
>
>Tietz, D.: J Chromatogr. 418, 305-344 (1987)
>
>Detailed procedures are given in:
>
>Diezel, W., G. Kopperschlaeger, G., and Hoffmann, E.: Anal.
>Biochem., 48, 617-620 (1972)
>
>Neuhoff, V., Stamm, R., and Eibl, H.: Clear background
>and highly sensitive protein staining with Coomassie Blue
>dyes in polyacrylamide gels: a systematic analysis.
>Electrophoresis 6, 427-448 (1985)
>
>Good luck!
>
>Dietmar Tietz
>
THANKS FOR THE REFERENCE, BUT CAN ANYONE SIMPLY SAY WHAT THE DIFFERENCE
BETWEEN COOMASSIE G AND R IS?  ALSO, WHY IS THE METHANOL CONCENTRATION SO
CRITICAL?? PLEASE DON'T JUST SAY "BETTER FIXING".



Marion S. Freistadt
Department of Microbiology, Immunology and Parasitology
Louisiana State University Medical Center
1901 Perdido St.
New Orleans, LA. 70112

504-568-4071
FAX-----2918




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