Thrombin cleavage problem

Graham Atherton grggta at picr.cr.man.ac.uk
Thu Aug 18 03:51:01 EST 1994


We are attempting to purify a protein expressed by the Pharmacia GEX
system. The protein expresses well and binds to the beads well when
purifying but once treated with thrombin to cleave the fused polypeptide
from our protein we get three or four bands of smaller MW than our
protein. It is thus possible that the thrombin is cleaving our protein
in several places?
Has this happened to anyone else and if so, what can be done?

Thanks
Graham Atherton
GRGGTA at picr.cr.man.ac.uk



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