qiagen plasmid preps

Artur Burzynski Artur.Burzynski at mcmail.vanderbilt.edu
Thu Aug 18 17:21:32 EST 1994


In article <Cuo0s2.F0J at mail.auburn.edu>, silverps at vetmed.auburn.edu (Peter S. Silverstein) says:
>
>There seems to be a lot of different opinions on Qiagen plasmid preps. 
>Does anyone have any experience using this system for transient 
>transfections?

I am doing transient transfections with Qiagene preps. It does work,
BUT the DNA must be "fresh" - stored at 4°C for a few days is no longer
good. I am freezing plasmid prep in aliquots sufficient for one exp
and it does work.

Artur Burzynski
Vanderbilt University



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