genomic DNA purification questions

Artur Burzynski Artur.Burzynski at mcmail.vanderbilt.edu
Thu Aug 18 17:17:14 EST 1994


In article <9408161553.AA13566 at post.its.mcw.edu>, FRED at bmt.mcw.edu ("Frederick Garbrecht") says:
>
>A neophyte (me) needs help.  When I am purifying genomic DNA from 
>human lymphoid cells, I have a lot of trouble in the 
>phenol-chloroform extractions - when I pipette off the upper aqueous 
>phase, the DNA strands always seem to be attached to the junk at the 
>interphase, so consequently I pull up a lot of the crap in the 
>interphase and can't seem to get rid of it.  Any suggestions?  Do 

You may try decrease DNA concentration (increase sample volume)

>people just take off half of the aqueous phase and sacrifice the rest 
>to get a clean preparation?  Thanks much --

Well, if you really want clean prep you must sacrifice some...

>
>Frederick C. Garbrecht
>fred at bmt.mcw.edu
>fgarbrec at post.its.mcw.edu
>Bone Marrow Transplant Program
>Medical College of Wisconsin
>phone 414 257 5053
>fax   414 257 7994


Artur Burzynski, PhD
Vanderbilt University



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