Coomassie Blue G-250 staining

Tracy Aquilla aquilla at salus.med.uvm.edu
Thu Aug 18 11:02:09 EST 1994


In Article <9408161749.AA11303 at unixsrv1.lsumc.edu>, mfreis at lsumc.edu (Marion
Freistadt) wrote:
>>> Does anyone have the information about using Coomassie Blue G-250 to
>>> stain protein gels?
>>>
>>> Please send your response to lin at mbimail.umd.edu
>>>
>>> Thanks.
>>You may find an overview in:
>>
>>Chrambach, A.: The Practice of Quantitative
>>Gel Electrophoresis, VCH Weinheim 1985
>>
>>Tietz, D.: J Chromatogr. 418, 305-344 (1987)
>>
>>Detailed procedures are given in:
>>
>>Diezel, W., G. Kopperschlaeger, G., and Hoffmann, E.: Anal.
>>Biochem., 48, 617-620 (1972)
>>
>>Neuhoff, V., Stamm, R., and Eibl, H.: Clear background
>>and highly sensitive protein staining with Coomassie Blue
>>dyes in polyacrylamide gels: a systematic analysis.
>>Electrophoresis 6, 427-448 (1985)
>>
>>Good luck!
>>
>>Dietmar Tietz
>>
>THANKS FOR THE REFERENCE, BUT CAN ANYONE SIMPLY SAY WHAT THE DIFFERENCE
>BETWEEN COOMASSIE G AND R IS?  ALSO, WHY IS THE METHANOL CONCENTRATION SO
>CRITICAL?? PLEASE DON'T JUST SAY "BETTER FIXING".
>
>
>
>Marion S. Freistadt
>Department of Microbiology, Immunology and Parasitology
>Louisiana State University Medical Center
>1901 Perdido St.
>New Orleans, LA. 70112
>
>504-568-4071
>FAX-----2918
>

    I think the only difference is in the purity of the dye, and the price
of course. Check in the Sigme catalog.

Tracy Aquilla, Ph.D.
Molecular Physiology and Biophysics
University of Vermont
aquilla at salus.med.uvm.edu



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