Qiagen/Nucleobond Cartridge Reuse
jwoodget at oci.utoronto.ca
Thu Aug 18 10:19:20 EST 1994
In article <CupCn3.5z9 at world.std.com>, nestgrp at world.std.com (Amos H
> Several of you have inquired as to why Qiagen or Macherey-Nagel suggest
> a limited period of time within which to reuse your cartridges for
> plasmid preps. This is the explanation for Nucleobond and since
> the products are nearly identical, separate patents not withstanding,
> the explanation will fit both.
> The products are silica based and as such the elution conditions of pH >
> 8.0 would dissolve the silica in the matrix upon long storage at
> these conditions. With the normal allowance for German
> English translation, the reasoning on a 3 hr or one day period is
> the following:
> 1. less than 3 hrs will assure silica will not have dissolved if
> you nonchromatographers have left the cartridges in the high pH buffer.
> 2. less than assumes that you washed the cartridges with at least 5
> column volumes of water and equilibrated the cartridges in the
> pH 6.3 buffer and then left the cartridges around. The one day
> would assure that the cartridges would not have dried out or formed
> air pockets which would prevent good access to the surface
> chemistry, which would result in lower yields.
> 3. you could wash the cartridges off with ethanol:water 50:50 and
> store them and when ready to reuse repeat the wash prior to
> equilibration with the binding buffer. This would prevent
> bacterial growth on storage, it would wet the surface for intimate
> contact of the buffer and would lower your cartridge consumption. Do
> you really think they want you to do that?
> If you want to try the Nucleobond, give me a call 800-347-6378.
> Amos (nestgrp at world.std.com)
Amos, despite your helpful comments you really should explicitly declare your
interest in these products as you are posting from a company that markets one
of them. This is standard netiquette and can help prevent misunderstanding,
conflict of interest, etc.
I might add that the two products you mention work equally well in our hands
and I am looking forward to some active competition to reduce the prices
further and thus extend my grants ;-)
(who has no affiliations with Diagen, Macherey-Nagel or the Nest Group)
P.S. Please don't use follow-ups to this message to increase your companies
exposure. I'm sure you will be a good net citizen from now on.......
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