E.coli transformation protocol wanted

Gabriel Dorado bb1dopeg at lucano.uco.es
Fri Aug 19 11:05:04 EST 1994


Actually, I just saw it in the last NAR issue --have not tried it yet!

It is based on the novel observation that cultures at high OD (0.94 -
0.95) do yield more transformants than when grown to classical OD (0.3
- 0.4). In short: 5 x10E7 - 1x10E9 vs 2.10E7 - 5x10E8 per microgram
DNA. Furthermore, you harvest competent bugs at 4 - 8 times higher
levels.

Hope it is useful!

G. Dorado
bb1dopeg at lucano.uco.es



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