Lux expression conditions??

MOUNCEY mouncey.mouncey-nfl at afrc.ac.uk
Fri Aug 19 08:48:59 EST 1994


Hi everyone,
  I'm using the luxCDABE genes from Xenorhabdus luminescens as a reporter for
expression from the bacterial promoter that I'm looking at from Azotobacter.
At the moment I'm looking at different conditions in E. coli to maximise the
levels of light produced. I'm wondering if anyone has any experience of lux as
a reporter and condtions (predominately growth condtions and optical density)
which maximise lux activity.
  Thanks
Nigel J. Mouncey
Nitrogen Fixation Laboratory
University of Sussex
Brighton
Sussex



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