ds Sequencing with Qiagen prep
Dr. C.J. Chan
cchan at hgmp.mrc.ac.uk
Mon Aug 22 09:20:50 EST 1994
I wrote to you all a couple of weeks ago about the problems that I had in
sequencing a Qiagen ds DNA prep. Thanks to all those people who replied
either directly to me directly or to the newsgroup.
I am glad to report now that my problem is solved! This template apparently
would only cycle sequence properly using radiolabelled primer but not using
the incorporation method. However, I have had many successes with other
templates using radioactive 35SdATP incorporation. So this appears to be a
template specific thing.
So if there are anybody out there who are having problems cycle sequencing
try end-labelled primers if incorporation does not work.
More information about the Methods