Plasmid size limitation on E. Coli Transformation?
BrianC4208
brianc4208 at aol.com
Mon Aug 22 14:28:10 EST 1994
I've been having a problem transforming DH5Alpha cells with a ligation
I've been trying. I think the problem might be that the plasmid is just
too darn big, somewhere < 15 KB. I hope someone has heard of a limit,
because I'm at a loss for any other explaination.
Thanks for any help you can lend.
Brian Cohen
Albany Medical College
brianc4208 at aol.com
More information about the Methods
mailing list