Plasmid size limitation on E. Coli Transformation?

BrianC4208 brianc4208 at aol.com
Mon Aug 22 14:28:10 EST 1994


I've been having a problem transforming DH5Alpha cells with a ligation
I've been trying.  I think the problem might be that the plasmid is just
too darn big, somewhere < 15 KB.  I hope someone has heard of a limit,
because I'm at a loss for any other explaination.
Thanks for any help you can lend.

Brian Cohen
Albany Medical College
brianc4208 at aol.com




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