Jeffrey Shane Temple
jstemple at whale.st.usm.edu
Mon Aug 22 14:27:21 EST 1994
Virginia Dress (Dress at biosci.arizona.edu) wrote:
: I need input on the Pichia expression system. I see lots of questions
: it but no answers. I would like to hear from people who have tried it, how
: well does it work? What size protein did you want to express? And other
: vital stats on the protein and how well the system worked for you. The
: expression system looks good on paper, but then, what doesn't?
: I'm pretty sure that this question has been posed before, so anybody who
: any answers -- I would appreciate hearing from you.
I have been working with the Pichia expression system now for about five
months and so far nothing. I am trying to express human Glutamine
Cyclotransferase (QC). This is a 33kD protein. I'm not sure if
there is something wrong with my construct or what, but I have been
unsuccessful thus far. Maybe something good will happen soon!! I feel
like you do, the kit looks good on paper, but I am not conviced yet.
Jeff (jstemple at whale.st.usm.edu)
More information about the Methods