plasmid recovery from "dead" plates
Kimberly Ann Winkeler
kwinkel at cc.emory.edu
Tue Aug 23 10:07:38 EST 1994
OK, I've tried several times to post this notice, and I apologize for all
the problems. One more time.
I am trying to recover some plasmids from what are apparently dead
colonies--colonies picked and inoculated into liquid media do not grow.
I have tried the Shuldiner and Tanner method for recovery of plasmids
from nonviable colonies (Biotechniques 12(1) p66 (1992)) which involves
picking a colony, boiling it in water, and transforming some of the
supernatant. I tried this method with four different clones with no
Has anyone tried this method (or any other method) for recovering
plasmids with any success? Does anyone have any tips or suggestions?
I'd really like to recover these plasmids if possible since trying to
reconstruct them will be a major pain in the butt!!!
Any and all help will be greatly appreciated.
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