PCR inhibitors in insect tissue?

edwardsg at skyfox.usask.ca edwardsg at skyfox.usask.ca
Tue Aug 23 11:24:49 EST 1994


Has anyone else experienced problems with PCR inhibitors when attempting to ampl
ify insect DNA.  I have tried GeneClean, Elutip-d columns, multiple phenol-chlor
oform treatments, however, some inhibitors remain and this really knocks down th
e sensitivity. If you have developed a new or old but inovative protocol please
let me in on it.



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