hplc column for peptide analysis

Paul Donald Pyla pdpyla at ccmail.monsanto.com
Wed Aug 24 11:00:29 EST 1994


In article <23AUG94.17510766.0062.MUSIC at SEMOVM>, C749SCB at SEMOVM.SEMO.EDU
(Walt Lilly) wrote:

> Dear Netters:
> We are trying to decide on an HPLC column to use for analyzing
> peptide fragments following proteolysis of a model peptide.  Will
> any old c18 column do?  We have been considering a Vydac 218TP
> column.  Does anyone have experience with this or competing similar
> columns?   Thanks for your input.
> 
>We have had good success with the vydac 218TP54 (5 micron particle) for
general proteolytic mapping.  Certainly, other columns can also be used. 
You should expect slightly different selectivity with other columns.



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