NA45 Paper DNA extraction problem

Carlisle Landel landel at helios
Wed Aug 24 14:15:04 EST 1994


>> In Article <317aqm$rp8 at matt.ksu.ksu.edu>, grieger at matt.ksu.ksu.edu (David M
>> Grieger) wrote:
>>
>>Netters,
>>
>>I had my student try to purify a 200bp fragment from a gel using the NA45
>>paper technique, recently discussed here on this news group.  After she did
>>the hi-salt washes and the EtOH precipitation (+glycogen), there was no
>>normal pellet but a nonaqueous "bubble" in the bottom of the microfuge tube.
>> After adding TE and doing a second EtOH precipitation the same result
>>occured.  Could this result from carry over from the hi-salt washes?  Any
>>thoughts on this problem would be appreciated.  Thanks.
>>
>>Beagle
>

Could you possibly have any phosphate buffer around?  Trying to EtOH ppt
from phosphate buffer also gives a funny "bubble" in the bottom of the tube.

Carlisle





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