Two-dimensional DNA electrophoresis

Richard Levenson levenson at duke.cs.duke.edu
Thu Aug 25 11:22:02 EST 1994


I have some experience with protein 2-D gel electrophoresis and was 
contacted by a colleague who needed help separating a complex mix of
PCR amplicons and who thought that a 2-dimensional approach might work.

There may be as many as 100-200 species all in the size range of
78-90 base-pairs in a single amplification reaction.  

Does any one have experience or ideas on how to demonstrate the
presence or absence of each species?  

We are considering a two-dimensional approach based on a denaturing
gradient gel in one dimension, followed by a sizing gel in the 
second dimension.  Thoughts on this strategy?

Thanks for your help.
~r .sig*
.



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