large plasmids

Rafa Maldonado Rafael at genetics.med.utah.edu
Thu Aug 25 19:02:48 EST 1994


In article <199408241657.AA17265 at wugate.wustl.edu>
brett at BORCIM.WUSTL.EDU writes:

> hi, our lab has encountered some stability problems with large (15+ kb)
> plasmids. Our solution is to use low copy number (ie pUC-derivatives) vectors
> and tet resistance, in MC1061 as host. You may want to give this a try.
> brett at borcim.wustl.edu

What? low copy number pUC? you must be kidding!
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Rafael Maldonado
Howard Hughes Medical Insitute
University of Utah
Rafael at genetics.med.utah.edu



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