help: phage DNA extraction
nash at nrcbsa.bio.nrc.ca
Thu Aug 25 17:26:42 EST 1994
In article <9408252046.AA00554 at leguin.cc.umanitoba.ca>,
<pzhang at CC.UManitoba.CA> wrote:
>I have extracted two batches of phage DNA from plate lysates. First batch
>was very good: yield was high, digestion was complete. However, second
>batch was not so good. DNA can not been digested completely. I did
>another phenol extraction and chloroform extraction. Thing gets worse: it
>The only difference between the two batches was that new plates were used
>with the first batch (2-day old plates for the first batch and 4-week old
>plates for the second batch).
You said it right there. In my hands, phage yields are always lousy
with old plates. I make 'em an hour before I use 'em.
John Nash | Email: nash at nrcbsa.bio.nrc.ca
Institute for Biological Sciences | http://cansnd.cisti.nrc.ca/~nash/home.html
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