Library screenings by PCR

Eric C. Anderson anderson at pharmdec.wustl.edu
Thu Aug 25 14:40:17 EST 1994


In article <9408251513.AA11622 at unlinfo.unl.edu>, ra at UNLINFO.UNL.EDU (raul
arredondopeter) wrote:

>      Do anybody know about application of PCR in library screenings? I
> shall appreciate any information available. Thank you in advance.
> 

i have screened a C. elegans Lambda-ZAP library by PCR using Inosine
containing degenerate oligos created based on conserved sequences in my
gene of interest from other species.  i performed the reactions just as i
would any other PCR reaction and was able to recover approximately 2/3 of
the coding sequence. 

i don't have any specific protocols to give you, but if you're interested i
can give you some suggestions and outline my PCR protocol.

eric

-- 
*************************************************
*there's too much blood in my caffeine system!  *
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eric c. anderson                                
anderson at pharmdec.wustl.edu
dept. of molecular bio. and pharm.               (314)362-3963 (lab)
washington univ. school of medicine              (314)862-2435 (home)
660 s. euclid box 8103                           (314)362-7058 (FAX)
st. louis, mo 63110



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