HPLC coloumn for peptides

berry at biochem.unp.ac.za berry at biochem.unp.ac.za
Fri Aug 26 07:55:51 EST 1994


I don't know how the hell this article got THIS title

Sorry ....

Ron

In article <33kmke$pf5 at owl.und.ac.za> berry at biochem.unp.ac.za writes:
>In article <23AUG94.17510766.0062.MUSIC at SEMOVM> C749SCB at SEMOVM.SEMO.EDU (Walt Lilly) writes:
>>Dear Netters:
>>We are trying to decide on an HPLC column to use for analyzing
>>peptide fragments following proteolysis of a model peptide.  Will
>>any old c18 column do?  We have been considering a Vydac 218TP
>>column.  Does anyone have experience with this or competing similar
>>columns?   Thanks for your input.
>>
>>Walt Lilly
>>c749scb at semovm.semo.edu
>>Southeast Missouri State Univ.
>
>
>
>Hello Walt
>
>Do yourself a favour and stick to a column designed for 
>peptide/protein analysis. We have found the Vydac 218TP columns 
>to be robust and reliable. Also the pore size plays a major 
>role in the separation of tricky peptide digests.  Ask you 
>Waters rep for a copy of "Doctor Tom's Guide to the 
>Undocumented Oral History of Peptide Separation Fokelore" - 9 
>pages of distilled wisdom!
>
>Good Luck
>
>Ron Berry
>Biochem Dept
>University of Natal
>Pietermaritzburg
>South Africa



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