Sma I degrading ends

Anthony Tomlinson anthony at pplros.demon.co.uk
Fri Aug 26 06:25:03 EST 1994


In article <940811144046.201d52 at thorin.uthscsa.edu> ,
HARDIES at THORIN.UTHSCSA.EDU writes:
>I am guessing that you detected the lost bases after transforming and 
>recovering a clone.  In that case, your problem may be at the ligation
>step.  If ligation fails, then you transform with the linear fragment
>and E. coli circularizes it by invading with one end at a target
>internal to the other (at low efficiency).  The result fits your 
>description.  
>

Is there any preference by the coli apparatus for certain sequences? How
does it know where the end is, and yet still invade a variable distance
from the end?  Sorry to ask dumn questions, but I've never heard of this
before, and it could explain a problem or two if mine.....

Thanks
Anthony



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