TAQ contaminated with DNA?
wkusser at sol.UVic.CA
Fri Aug 26 12:17:06 EST 1994
got rid of DNA contamination problem by purifying Taq over a Heparin Sepharose column (thinking that Taqwith bound DNA will not bind to the column). A mini column with a low salt wash and elution of Taq with .55 M KCl does the job and is pretty fast. More details on buffers etc. can be found in PCR Methods and Application 2:275-285 (1993).
Wolfgang Kusser Univ. of Victoria Dept. of Biology Centre for Env. Health
More information about the Methods