TAQ contaminated with DNA?

Wolfgang Kusser wkusser at sol.UVic.CA
Fri Aug 26 12:17:06 EST 1994


 got rid of DNA contamination problem by purifying Taq over a Heparin Sepharose column (thinking that Taqwith bound DNA will not bind to the column). A mini column with a low salt wash and elution of Taq with .55 M KCl does the job and is pretty fast. More details on buffers etc. can be found in PCR Methods and Application 2:275-285 (1993). 
Wolfgang Kusser Univ. of Victoria Dept. of Biology Centre for Env. Health



More information about the Methods mailing list