e.coli transformation question

Andreas Weber aweber at biolan.uni-koeln.de
Fri Aug 26 08:41:43 EST 1994



Did somebody try the transformation protocol described in
Meth. Enzymol. 218: 621-627 (1993, Chung a. Miller) ? It is a
one step procedure using TSS-buffer (PEG,DMSO,Mg2+ in LB) and authors
claim to get 10E7-10E8 /yg DNA.

Thanks in advance

Andreas

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* Andreas Weber                    Tel.: (49)-221-470-5664  *
* University of Cologne            Fax.: (49)-221-470-5181  *
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