Growing Up TA Cloning Products
AMCARTHU at UVVM.UVIC.CA
Sat Aug 27 17:09:16 EST 1994
Hello everyone. For those of you that use the TA cloning kit (Invitrogen),
what is your prefered method for overnight cultures of your cells with
inserts? I have been using an overnight in LB (with ampicillin) but have
not been getting a satisfactory amount of plasmid DNA. Has anyone tried
TB media or chloramphenicol with this kit to increase plasmid DNA yield.
Much thanks for your comments.
More information about the Methods