Growing Up TA Cloning Products

Andrew McArthur AMCARTHU at UVVM.UVIC.CA
Sat Aug 27 17:09:16 EST 1994


Hello everyone.  For those of you that use the TA cloning kit (Invitrogen),
what is your prefered method for overnight cultures of your cells with
inserts?  I have been using an overnight in LB (with ampicillin) but have
not been getting a satisfactory amount of plasmid DNA.  Has anyone tried
TB media or chloramphenicol with this kit to increase plasmid DNA yield.
Much thanks for your comments.

Andrew



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