Hints for low-stringency cloning

Tae Bum Shin tshin at fas.harvard.edu
Sat Aug 27 19:22:47 EST 1994


Hi there;
	We're currently trying to clone a homologue of a gene by using 
the original cloned gene as a template for a random-primed probe.  Does 
anyone have any hints or suggestions on hybridization/washing 
conditions?  

Thanks;

T.B. Shin
tshin at fas.harvard.edu





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