Subcloning dirty PCR products

Tomas Bratt TOMAS.BRATT at MEDKEM.LU.SE
Mon Aug 29 09:58:13 EST 1994


Subject: Subcloning dirty PCR products
From: rosas



I have had the same problem with my PCR-products for over four months.
After advice from PCR-experts, I treated the fragments with klenow DNA
polymerase. Appearently some RE are sensitive to Taq-produced ends and
will not cleave. It worked for me.
Good Luck

Tomas



More information about the Methods mailing list