Growing up TA cloning products

Graham Atherton grggta at
Mon Aug 29 03:15:01 EST 1994

We have a few constructs based on pBR. We find that growing in Terrific
Broth in a baffled flask using Carbenicillin instead of ampicillin
(see Maniatis for concentration) and growing for 36 hours rather than 
just overnight gives mg of DNA per litre bugs (usually!), with no
apparent problems. Quicker than subcloning?
Graham Atherton

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