Hints for low-stringency cloning

Doc Martin pkmartin at netcom.com
Mon Aug 29 19:58:30 EST 1994


Tae Bum Shin (tshin at fas.harvard.edu) wrote:
: Hi there;
: 	We're currently trying to clone a homologue of a gene by using 
: the original cloned gene as a template for a random-primed probe.  Does 
: anyone have any hints or suggestions on hybridization/washing 
: conditions?  

: Thanks;

: T.B. Shin
: tshin at fas.harvard.edu


tae...

i'm surprised at you.  start with maniatis an use your head.

say hi to lynn z. for me.

pat martin




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