membrane preparation from COS cell

Georges Gaudriault mazella at naxos.unice.fr
Tue Aug 30 10:37:43 EST 1994


Hi netters,
I've transfected COS cells with pcDNAI plasmid and would like to prepare
membrane for binding studies. Everything seems to be normal but i've to
attach a big pellet of DNA. The studied protein is very sensitive to
sonication and to protease so i could only treat my lysed cells at 4=B0C. At
this temperature,
DNAse I doesn't work. So my question is : HELP !!!!

What could i do to destroy this DNA ?

Thanks by advance for response

Georges



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Georges GAUDRIAULT
Institut de pharmacologie moleculaire et cellulaire
660, route des lucioles
06560 valbonne
=46RANCE
Tel:19 (33)-93-95-77-61
Tel:19 (33)-93-95-77-08
e-mail :mazella at naxos.unice.fr
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