large plasmids

Tracy Aquilla aquilla at salus.med.uvm.edu
Tue Aug 30 09:52:00 EST 1994


In Article <199408241657.AA17265 at wugate.wustl.edu>, brett at BORCIM.WUSTL.EDU
wrote:
>hi, our lab has encountered some stability problems with large (15+ kb)
>plasmids. Our solution is to use low copy number (ie pUC-derivatives) vectors
>and tet resistance, in MC1061 as host. You may want to give this a try.
>brett at borcim.wustl.edu

You may also want to try SURE cells (Stop Unwanted Recombination Events)
from Stratagene.

Tracy Aquilla, Ph.D.
Molecular Physiology and Biophysics
University of Vermont
aquilla at salus.med.uvm.edu



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