direct sequencing from chromosome?

adler at staff.monash.edu.au adler at staff.monash.edu.au
Tue Aug 30 01:46:58 EST 1994


Has anyone out there tried direct automated cycle sequencing (we have an 
Applied Biosysytems setup) on bacterial chromosomal DNA? We have some sequence 
from a partial clone (hence the primer), but are having a devil of a time 
cloning the remainder. Any experiences (e.g. what DNA concentration is 
really necessary) would be very much appreciated.
Many thanks
Ben Adler
adler at ccs1.cc.monash.edu.au




More information about the Methods mailing list