direct sequencing from chromosome?

adler at adler at
Tue Aug 30 01:46:58 EST 1994

Has anyone out there tried direct automated cycle sequencing (we have an 
Applied Biosysytems setup) on bacterial chromosomal DNA? We have some sequence 
from a partial clone (hence the primer), but are having a devil of a time 
cloning the remainder. Any experiences (e.g. what DNA concentration is 
really necessary) would be very much appreciated.
Many thanks
Ben Adler
adler at

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