PCR from intact bacterial cells

Rachelle Leger ax041 at FreeNet.Carleton.CA
Wed Aug 31 18:43:17 EST 1994


In our lab we routinely screen our colonies by picking it and 
dispensing it in 50 microliters of water.  After vortexing it,
we use one microliter for the PCR reaction.  After the PCR and
running the gel we use the 50 microliters containing the bacteria
to grow in 5 ml of LB for next day minipreps. The first step of
denaturation of the PCR (3min, 94 degrees) effectively bursts
open the bacteria.

Good luck!

Rachelle
email: leger at medcor.mcgill.ca



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