long range PCR

m-brazil at nimr.mrc.ac.uk m-brazil at nimr.mrc.ac.uk
Wed Aug 31 10:42:29 EST 1994

Hi everybody,

I have seen adverts for Taq extenders, even up to 40kb. Has anybody reached
these lengths?

I also remember some discussion about making your own taq extender. Could
someone remind me how to do it? Sorry for not paying attention before!!

Regarding primers with a mismatch at the 3' end. Has anybody used this
method to distinguish between the presence (or absence) of point mutations?
Any tips greatly appreciated.


(m-brazil at uk.ac.mrc.nimr)

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