How to treat cells in interphase stage for FISH?
asmsi002 at CMU.CHIANGMAI.AC.TH
Wed Aug 31 10:06:10 EST 1994
We have successfully used WCP system (Whole Chromosome painting Probe)
either from LTI or Imagenetic, Inc for identifying chromsome in metaphase
spreads from lymphocytes. However, we do not have any luck to detect any
chromosome from cells in interphase stage, such as human buccal mucous
Does anyone out there would kindly provide us a nice protocol
to treat cells other than lymphocytes, particularly human buccal mucous
membrane cells prior to perform FISH?
Wasun Chantratita, Ph.D.
Department of Clinical Microbiology
Faculty of Associated Medical Sciences
Chiang Mai University
Chiang Mai 50200
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