Methods August 1994 Archives by date

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Starting: Mon Aug 1 03:48:24 EST 1994
Ending: Wed Aug 31 22:44:08 EST 1994
Messages: 1080

"sticky" sequencing gels jenkins at aidsun.nibsc.ac.uk
antibody production Clemens Suter-Crazzolara
NM1149 cloning site sequence? Frances Hannan Zoology
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
Sequence or Rest. Map for pGP1-2 Christopher Walentas Bioc
Sequence or Rest. Map for pGP1-2 Robert Solomon Bioc
Advice on ABI373A Sequencing Conditions? Bruce Roe
Help needed about Branson Sonifier! Inst. f. Biochemie
Non EtBr DNA stain(s) Andrew Cockburn
Universal PCR Dick Beeman
Gel Purification of DNA with Qiaex beads Clemens Peterbauer
EMSA-Competition problem Peter Brunovskis
Ni/His-tag 4 affinity purification of ABs? Anne Spang
PCR and Strand Displacement Parke K. Flick
Dissuruption Buffer szcooley at chip.ucdavis.edu
More GelReader Manuel G. CLAROS
Pointer to Biologist's Internet Resource Guide Requested Susan Kretschmer
BTX electroporators--info please? ALLEN, BEVERLY S
sequence software Seth Daniel Crosby
Lost DNA after precipitation Zengjian Chen
PCR woes Purnima Laud
mammalian expression vector Seth Daniel Crosby
EMSA-Competition problem gc
Oligo degradation trepidation szcooley at chip.ucdavis.edu
Qiaex problems (was Re: Gel Purification of DNA with Qiaex beads) neale at mbcf.stjude.org
Oligo degradation trepidation Thomas M. Rupp
***PLEASE HELP with NADH*** X-Man
In situ hybridization of cell suspensions? Bohdana Badzio
Custom baculoviral expression services Garry Myers
Dissuruption Buffer daj (David Johnston)
Transformation method for Rhodobacter capsulatus? Mareike Trieschmann
Kit for sequencing mRNA Henrik Wengholt
Non EtBr DNA stain(s) Sebastian W. Bunka
Anyone have a spare cell disruptor? Brian T. Greuel
RNA isolation Brett Beitzel
mammalian expression vector pskaytes at INTNET.UPJ.COM
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
Reused Electroporation Cuvettes? George W Chacko
mammalian expression vector Clemens Suter-Crazzolara
Modified dNTPs Gerri Newfry
help with in-situ hyb. zamir at agri.huji.ac.il
Universal PCR Doug Rhoads
Lipid Peroxidation Stains for EM histology Gary Krause
Dissuruption Buffer dpetersn at unixg.ubc.ca
pI of viral protein AKEY HUNG
Universal PCR Dick Beeman
Silver staining of DNA (help) Larry Hale
Biologist's Internet Resource Guide Emma Macfarlane
HELP!!! PCR Smear! Brian Foley
sequence software Gill Diamond
Fixing sequencing gels Kilroy, Gail - Dr. York 2545
Advice on ABI373A Sequencing Conditions? Steve Marsh
Reused Electroporation Cuvettes? Dick Vogt
Monocyte blues: help! tronni at ktl.fi
Need RNA Isolation Protocol Jacqueline J Janka
Reused Electroporation Cuvettes? szcooley at chip.ucdavis.edu
SURAMINE Vuk Stambolic
Pointer to Biologist's Internet Resource Guide Requested Christopher B. Yohn
sequence software Robert Woodward
Chromosome probes for ISH Marie-Terese Little
subscribe Eduardo A. Garcia-Zepeda 7356000
RNA labeling with pCp and ligase ? Frank Ma
half-life of mRNA in bacteria (specifically Psuedomonas aeruginosa) hallinanp at topaz.cqu.edu.au
DNA Fingerprinting U27111 at uicvm.uic.edu
Baculovirus Jane Harrison
Silver staining of DNA Mike Morris
HELP!!! PCR smears! Bengt Oxelman
in vitro transcription question Paul Thompson
About 32PdCTP conc. in quantitative PCR 石塚俊治
'Leaky' IPTG inducible promoters... Simon Twigger
pulse-chase w/T7 system Julie Forney
Variability Between Plasmid Preps in Transfections Yun-Long Du
leaky IPTG inducible promoters Emma Macfarlane
RNA gels: Autoclaving MOPS buffer? herzer at urz.unibas.ch
particle-gun eric c. anderson
RNA gels: Autoclaving MOPS buffer? Carlisle Landel
Silver staining of DNA dkim at unm.edu
'Leaky' IPTG inducible promoters... Bernard Heymann
Reused Electroporation Cuvettes? Nancy Lemieux
Split up methods-reagents? Bert Popping
genbank submission Marion Freistadt
recycle GST-agarose beads? shiha02 at mcclb0.med.nyu.edu
Perfusing a mouse John Watson
Kan vs Amp for eukaryotic vectors ? John Watson
Reference Manager vs Endnote John Watson
Variability Between Plasmid Preps in Transfections Brian Foley
antibody production Jello ease
Alpha-1-pi Elisa protocol wanted kathy frees
summary of mycoplama detect Patrick Grealish
RT PCR-any suggestions? Tracy Aquilla
Streptavidin-coated microtiter plates walling at wmvx.lvs.dupont.com
PFGE Southern Blots Panhead McNipper
in vitro transcription question Virginia Dress
particle-gun walling at wmvx.lvs.dupont.com
in vitro transcription question Bohdana Badzio
silver staining of DNA Allergan
genbank submission John Watson
UV-transparent microtiter plates John H McDonald
Large-scale DNA cleanup szcooley at chip.ucdavis.edu
Glass Matrix MBPI at BIOTECHNET.COM
RNA gels: Autoclaving MOPS buffer? David Glover
split up/sequencing gels Patricia Foster
Ecdysone receptor William W.-G. Jia
RNA gels: Autoclaving MOPS buffer? Aaron Pawlyk
in vitro transcription question Aaron Pawlyk
Reused Electroporation Cuvettes?
PFGE Southern Blots Antje Haase
RNA gels: Autoclaving MOPS buffer? Kelly Tetro
CIPPers for breakfast Pendragon
Plasmid Prep Scale-up Pendragon
Split up methods-reagents? Pendragon
Fixing sequencing gels Pendragon
Lost DNA after precipitation Pendragon
High level protein expression MMKania
HELP! How to kill phages? Troels Wind
Cloning into Afl III site in pUC based plasmids Duncan Clark
UK distributor for Branson Sonicators Duncan Clark
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
RT PCR-any suggestions? Too Heng-Phon
RNA gels: Autoclaving MOPS buffer? Too Heng-Phon
Transformation advice needed? Me too! Sue Jeffrey
RNA gels: Autoclaving MOPS buffer? Tom Thatcher
PAGE gels cracking Jamie Cope
RNA gels oesch at hifo.unizh.ch
'Leaky' IPTG inducible promoters... John McDougall
Autoclaving MOPS buffer Dr. C.J. Chan
Transformation advice needed? Me too! Stuart Kuhstoss
Amersham site mutagensis kit? Yi Dou
rat random cDNA ??? K.D.Preuss
Transformation advice needed? Me too! Stefan Kahlert
Ultralow recommendations needed Shiao Y. Wang
Baculovirus Sean David Moore
DNA Fingerprinting Dave
RT PCR-any suggestions? Annette C. Hollmann
RT PCR-any suggestions? Hanson Lab
Agarose-PAGE Gels Scott Selby Med Heme
Silver Staining of DNA Allergan
Detergent Quantification fintan_v at Msu.oscs.montana.edu
CIPPers for breakfast Carlisle Landel
Monocyte blues: help! Ben Vowels
Help? Silver Stain DNA sequencing. Yi Dou
metal probe BTC-5N ez041225 at chip.ucdavis.edu
mammalian expression vector SYE at OPUS.MCO.EDU
Protein Phosphatase 1 or 2A cDNA clone LONG,Benedict
Pointer to Biologist's Internet Resource Guide Requested Christopher B. Yohn
Cloning into Afl III site in pUC based plasmids Ward Lab
Custom baculoviral expression services Steve Rodems
Baculovirus Steve Rodems
Wanted: Ferredoxin Assay dmncli02 at uctvax.uct.ac.za
coli RNA polymerase F.J.van der Wal
Dissuruption Buffer daj (David Johnston)
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
german whatman 3MM equivalent Clemens Suter-Crazzolara
Lost DNA after precipitation schupp at nauvax.ucc.nau.edu
PCR woes schupp at nauvax.ucc.nau.edu
split up/sequencing gels Zophonias Jonsson
'Leaky' IPTG inducible promoters... Duncan Clark
genbank submission Paul Riggs
373A Automated Sequencer etc... ir (Ian Ridgers)
glycogen in sequencing rxns??? russos at HAL.HAHNEMANN.EDU
unsubscrib HERBERT WYCKOFF
unsubscribe HERBERT WYCKOFF
Biologist's Internet Resource Guide Una Smith
Biologist's Guide IS on WWW Una Smith
DNA (oligo manufacturers) Joe M. SandyFaulkner, Jr.
E Mal il and methods and reagents MBPI at BIOTECHNET.COM
Cycle sequencing of PCR products Tony Kwan
Kunkel Oligo-Directed Mutagenesis Chris Greene
373A Automated Sequencer etc... eric c. anderson
Sequencing from boiling mini-preps? Jean Valjean
Ultralow recommendations needed szcooley at chip.ucdavis.edu
Tips for 12 chan. multi-pipette David MacHugh
Silver Staining of Sequencing Gels Allergan
Ultralow recommendations needed Lisa J Brunet
373A Automated Sequencer etc... Dave Dudley
Reused Electroporation Cuvettes? Jon Visick
reusable sponges for southerns stewart at camis.stanford.edu
Lost DNA after precipitation Annette C. Hollmann
glycogen in sequencing rxns??? Domingos henrique
_E. coli_ 13706???? Jamie Stanek
RNA gels Dr. G. Koraimann
Silver Stained Sequencing Gels and EDF Film Development Allergan
SYMBR Green 1 carlos h. pedemonte
Ligand Pharm. email address? Doc Martin
HELP! Need to make ultra-complex plasmid library Ben-Sapiens
BTC-5N metal probe (re-post) ez041225 at chip.ucdavis.edu
Scanning Cell Culture Colonies Kim Konrad
** Help with AO staining of Drosophila salivary glands ** Sinisac Mike
Gel Purification of DNA with Qiaex beads Marion Freistadt
PCR detection Ian Dunn
Best Way to Sequence PCR Products? gustilo at pobox.upenn.edu
Protein Phosphatase 1 or 2A cDNA clone John Watson
PCR woes Satyam Subrahmanyam
Biotin and BCA protein assay! Prachya Kongtawelert
More GelReader Anthony Tomlinson
german whatman 3MM equivalent Robert Slany
Sequencing from boiling mini-preps? Bruce Roe
Protocol for Fluorescently Labeling Antibody Wanted Albert Smith
Osmotic Lysis of Erythroid cells - help wanted! Mr. A.F. Hill
More GelReader Manuel G. CLAROS
Journal: Molecular & Cellular Probes - anyone have Email address? Peter M. Muriana
Cycle sequencing of PCR products Frank White
Best Way to Sequence PCR Products? Frank White
DNA Fingerprinting Orange County Sheriff Forensic Science
Sequencing from boiling mini-preps? John Nash
Protocol for Fluorescently Labeling Antibody Wanted Flip Hoedemaeker, CvF RUL/TNO, Leiden, The Netherlands
<None> sstrain_ssb at opal.tufts.edu
PC-12 sub-clones needed sstrain_ssb at opal.tufts.edu
Quantification on phosphorimager Elizabeth Rosenberg
Quantification on phosphorimager Elizabeth Rosenberg
DNA Fingerprinting U27111 at uicvm.uic.edu
SUMMARY: Help on DNA quantification and RAPD... Naresh C. Pancholi
SUMMARY-2: Help on DNA quantification and RAPD... Naresh C. Pancholi
Two-subunit luciferase in yeast expression vector? Martin Latterich
antibody production Peter J. Crilly
DNA (oligo manufacturers) Robert Horton
help on LacZ needed... tao at mbcf.stjude.org
PNGaseF SPAGNOL at PLAUTO.CSATA.IT
Cycle sequencing of PCR products hpotter at chmeds.ac.nz
microsatellites in seattle Beth Karen Wapelhorst
DNA Fingerprinting Barbara Greene
codon usage Database? Dave Pilgrin
Wanted, Rabbit Muscle cDNA library!! Peter Kristensen
codon usage Database? Peter Weber
RNA extraction from plants? SUSI
Sequencing from boiling mini-preps? Greg Gloor
fragment isolation w/o glass milk? Daniel Schlieper
Ultralow recommendations needed schupp at nauvax.ucc.nau.edu
Cycle sequencing of PCR products schupp at nauvax.ucc.nau.edu
disappearing bands Frederick Garbrecht
simple method for sequencing from boiling mini-prep Chuck Miller
Reused Electroporation Cuvettes? eric c. anderson
Ultralow recommendations needed Bert Popping
SYBR Green 1 Michael van Waes
Gel Shift Assay--Probe in Wells Peter Brunovskis
DNA Fingerprinting Dana Macelis-Roberts Lab
fragment isolation w/o glass milk? John Brunstein
Non-fixing and non-drying sequencing gel Ho-Hyung Woo
E.coli strains with no lacZ, anyone have such a beast? tao at mbcf.stjude.org
373A Automated Sequencer etc... Mr V. Schoenfeld DOM
Best Way to Sequence PCR Products? Mr V. Schoenfeld DOM
Cycle sequencing of PCR products Mr V. Schoenfeld DOM
Degenerate PCR Sean David Moore
subscribe Allergan
E.coli transformation protocol wanted ez003450 at chip.ucdavis.edu
disappearing bands Aaron Pawlyk
Carbonate Extraction Rizwan Haq
Cycle sequencing of PCR products (fwd) Robert Preston
Altered Sites -Pros? Cons? jcuozzo at opal.tufts.edu
Gel Shift Assay--Probe in Wells gc
Gel Shift Assay--Probe in Wells Steve Rodems
Licensed Taq prices Paul A. Vescio
Licensed Taq prices Duncan Clark
_E. coli_ 13706???? Duncan Clark
Sequencing from boiling mini-preps? berry at biochem.unp.ac.za
Automated sequencing Graham Atherton
Plant Nucleic Acid Isolation Richard Dana
Reference Manager vs Endnote Ola Myklebost
cellulose acetate gel electrophoresis AMRUT S BHOGLE
Altered Sites -Pros? Cons? douglas gray cancer research
Gel Purification of DNA with Qiaex beads Anthony Tomlinson
TRUE OR FALSE? SPAGNOL at PLAUTO.CSATA.IT
Slot-Blot quantification of abundant mRNAs Allison Hanly
E.coli strains with no lacZ, anyone have such a beast? szcooley at chip.ucdavis.edu
DNA extraction from pollen szcooley at chip.ucdavis.edu
c-src T. S. Pillay
PCR thermal cyclers. Peter J. Crilly
9-cis retinoic acid sources needed Gary L. McKnight
Fixing sequencing gels cdickel at eye1.eye.ufl.edu
fragment isolation w/o glass milk? nishir at ohsu.edu
test frandenm at TCPLINK.NREL.GOV
Non-isotopic detection of nucleic acids Peter Leif Bergsagel
muscle protein extracts Jodie Mangor
E.coli strains with no lacZ, anyone have such a beast? Mark Farinha
Gel Shift Assay--Probe in Wells Michael Robert Ladomery
non-radioactive detection? AARON J. MOE
fragment isolation w/o glass milk? Sean David Moore
terminal transferase & PCR, Brian Foley are you listening? Zophonias O. Jonsson
ERBB2 Muriel Liberto
codon usage Database? Michael Coyne
non-radioactive detection? John Nash
PCR thermal cyclers. Daniel P. Kolk
Poly dT cellulose nika
NIH Image to read sequencing gels? Craig E. Cameron
Sequencing from boiling mini-preps? Tracy Aquilla
DNA (oligo manufacturers) Tracy Aquilla
codon usage Database? Tim Buss
in vitro transcription question Tracy Aquilla
'Leaky' IPTG inducible promoters... Tracy Aquilla
RNA gels: Autoclaving MOPS buffer? Tracy Aquilla
Baculovirus Tracy Aquilla
non-radioactive detection? Andre Hamel
non-radioactive detection? Andre Hamel
Osmotic Lysis of Erythroid cells - help wanted! DjayR
Lambda gt library D.J. WISE
non-radioactive detection? John Nash
Fixing sequencing gels Nicola Fidler
Licensed Taq prices Frank White
need help in analysing fatty acids rgroup at aardvark.ucs.uoknor.edu
DNA Fingerprinting Jason Fawcett
Best way to size select cDNA ? Dave Smith
SDS removal agmclen at liv.ac.uk
Long Ranger sequencing gels Bengt Oxelman
Common Forward Primers Ruari MacDonald
Long Ranger sequencing gels Paul A. Vescio
Azure C nucleus stain Yoshiki Kawabe
Automated sequencing John E. Fox
Cycle sequencing of PCR products (fwd) Mr V. Schoenfeld DOM
help on LacZ needed... Paul Riggs
stripping Western blots Peggy Halleck
BTC-5N metal probe (re-post) Raffaele Matteoni
Methods for RNA purification? Hubert Forkl
DNA (oligo manufacturer Margaret Shirra
inquiry: base-pair stability constants Erik Forbes Y. Hom
Autoclaving MOPS buffer/TRIS buffer dkim at unm.edu
Bact. luciferase reporter vectors schupp at nauvax.ucc.nau.edu
stripping Western blots FJ van der Wal
Automated sequencing eric c. anderson
Maize cDNA Library Wanted enyedi at wmich.edu
To Help or Not To Help Steve Konings
Gelatin as blocking agent in Westerns Peter Heifetz
Quantitation of small amts of DNA Richard R. Hardy
one-step RNA isolation reagents Amanda Hayward-Lester
DNA (oligo manufacturer Jack M. Bernstein, M.D.
TRUE OR FALSE? David L. Haviland, Ph.D.
Long Ranger sequencing gels Mark Farinha
help: leaky expression vector? Knaebel David B
Transformation advice needed? Me too! Song Tan
Best way to size select cDNA ? Thomas H. Rude
To Help or Not To Help Ron Kagan
spectinomycin amplification of plasmid Joe M. SandyFaulkner, Jr.
Shoddy Ann Arbor Plastics Products. Stephen R. Lasky
Non-isotopic detection of nucleic acids Klaus Salger
Shoddy Ann Arbor Plastics Products. Ron Kagan
genbank submission Diana Airozo
Illigitimate/Ectopic Transcription Carla Drebing
Nucleic Acid Purification MBPI at BIOTECHNET.COM
Non EtBr DNA stain(s) Paul N Hengen
High Five cells Robert C. Colgrove
RNA gels: Autoclaving MOPS buffer? neeraj jain
Non-isotopic detection of nucleic acids Scott Selby Med Heme
stripping Western blots Scott Selby Med Heme
Oligo Cloning Ben-Sapiens
Inoue Transformation Method Ben-Sapiens
Mwatson's 20 kbp PCR question. Tracy Aquilla
NA45 Paper DNA extraction problem Tracy Aquilla
F' restriction sites Patricia Foster
Sma chewing back - suggestions? Dr S T May
373A-stretch survey hollingswor at immunex.com
Altered Sites -Pros? Cons? Simon E Plyte
Quantitation of small amts of DNA Nicola Fidler
** Help w/ AO staining of drosophila salivary glands Sinisac Mike
Long Ranger sequencing gels Brandon S Johnson
[Q]cycle sequencing Toshio Ikeda
Quantitation of small amts of DNA John Watson
High Five cells Cornelius Krasel
help: leaky expression vector? FJ van der Wal
Gelatin as blocking agent in Westerns awb at aber.ac.uk
Strand Displacement Amplification Duncan Clark
3 PRIME AND 5 PRIME RACE JŸrgen Wendland
RNA gels: Autoclaving MOPS buffer? Jim Owens
ligation not working? Tomas Bratt
DNA from Pollen LONSDALE
plasmid that won't cut Hanson Lab
Annealing Temperatures for PCR rosas at mcclb0.med.nyu.edu
Modified dNTPs ott at wmvx.lvs.dupont.com
Non-isotopic detection of nucleic acids walling at wmvx.lvs.dupont.com
Sequencing plates Ian Wood,SR306,3632
one-step RNA isolation reagents contente at usuhs.usuhs.mil
stripping Western blots walling at wmvx.lvs.dupont.com
Gelatin as blocking agent in Westerns Andre Hamel
What's the L8 mutation in lac-Prom. Frank Buchholz
Gelatin as blocking agent in Westerns frandenm at TCPLINK.NREL.GOV
Annealing Temperatures for PCR fgrun
3 PRIME AND 5 PRIME RACE John Watson
Annealing Temperatures for PCR John Watson
Annealing Temperatures for PCR Clemens Suter-Crazzolara
Radioisotope Joe M. SandyFaulkner, Jr.
sma I chewing back - any suggestions? txpljfg at UABCVSR.CVSR.UAB.EDU
non-radioactive detection? walling at wmvx.lvs.dupont.com
The roles of gelatin, tween20 and glycerol in PCR Alex Chang
Oligo Manufacturers Joe M. SandyFaulkner, Jr.
Semi dry blotting caution! Dennis Templeton
EIA software? 93huebner at wmich.edu
Digene Fastpair B Stickney
Gel Purification of DNA with Qiaex beads neale at mbcf.stjude.org
Sma I degrading ends HARDIES at THORIN.UTHSCSA.EDU
chloroplasts & rho factor? Bob Gross
Sequencing plates glover martin
Gel Purification of DNA frandenm at TCPLINK.NREL.GOV
test Stringer, Peta
Tnt system-in vitro translation T. S. Pillay
Qiagen Plasmid Preps kalmar at sfu.ca
drug selection on CHO cells David Char
HSV-1 VP16 antibody? PETER PECHAN
373A-stretch survey Steve Marsh
Split up methods-reagents? Bernard Murray
? Swiss Protein - kw searches Tony Long
Removing ssDNA from dsDNA - help Joe DeRisi
Split up methods-reagents? m.c. evers
sequencing kits? David M Grieger
EIA software? Sorin Damien
ELISA for TRH Robert Bateman
Removing ssDNA from dsDNA - help Scott B. Mulrooney
Quantitation of small amts of DNA Anita Gould
Origin of C. elegans Rafa Maldonado
Address needed from Syntex Research, Palo Alto! Inst. f. Biochemistry
Address from Syntex Research, Palo Alto needed! Inst. f. Biochemistry
Annealing Temperatures for PCR Paul A. Vescio
PCR thermal cyclers. Andy Law Big Nose
SDS removal Timothy Bushnell
erbB2 gene Muriel Liberto
Annealing Temperatures for PCR schupp at nauvax.ucc.nau.edu
Oligo Manufacturers schupp at nauvax.ucc.nau.edu
Address from Syntex Research, Palo Alto needed! Robin Ann Wasserman
Scanning Cell Culture Colonies TJR
ELISA for TRH Robert Bateman
plasmid that won't cut HARDIES at THORIN.UTHSCSA.EDU
FAX No. for Branson Sonicators Duncan Clark
High Five cells dallas at mcvax4.d48.lilly.com
Sequencing plates Tanya Osickey
Shoddy Ann Arbor Plastics Products. Chris Noren
Storage for PCR Dan McGoldrick
The roles of gelatin, tween20 and glycerol in PCR Alex Chang
Biodegradable substance Karine Gabrielian
annealing temps. for PCR HARDIES at THORIN.UTHSCSA.EDU
Peptide mapping Ravi Ramachandran
Disappearing Plasmids--Help! Carlisle Landel
ds Sequencing Qiagen-prep Plasmids-Help! Carlisle Landel
chloroplasts & rho factor? Bob Gross
Silver staining of DNA (help) micha at myhost.subdomain.domain
Storage for PCR Dan Oerther
Strand Displacement Amplification Dick Beeman
Tnt system-in vitro translation Steve Rodems
Test lq at dna.warwick.ac.uk
E.coli transformation protocol wanted Gabriel Dorado
Split up methods-reagents? Anne Spang
ds sequencing Quiagen plasmid preps plxigpg at vax.ccc.nottingham.ac.uk
Origin of C. elegans Stephen Nurrish
terminal transferase & PCR, Brian Foley are you listening? Brian Foley
To Help or Not To Help Yasha Hartberg
Deiodinase Type II cDNA Aaron Pawlyk
Gelatin as blocking agent in Westerns Shahram Mori
Oligo Manufacturers Shahram Mori
High Five cells Harry Menegay
Pfu Poly, Cloning ez001427 at dale.ucdavis.edu
ligation not working? Brian Foley
Annealing Temperatures for PCR Brian Foley
Disappearing Plasmids--Help!
Shoddy Ann Arbor Plastics Products. Stephen R. Lasky
Fixing sequencing gels Yasha Hartberg
RNA from skin + uterus, protein contamination woes acer
Perkin Elmer Gene AMP? Shahram Mori
TnT system in vitro translation Stefan Gruenert (WCI)
Kunkel Oligo-Directed Mutagenesis Iain Wilson
Quick Coomasie Destain Allison Haggarty
Pfu Poly, Cloning Hong Dang
The roles of gelatin, tween20 and glycerol in PCR szcooley at chip.ucdavis.edu
PCR to differentiate meats Viraj Master
Current Contents online Duncan Clark
Expression in Pichia? f_benson at icrf.icnet.uk
Degenerate PCR Yasha Hartberg
Origin of C. elegans eric c. anderson
TGF-beta expression vector wanted G Gallagher
inquiry: base-pair stability constants Greg Gloor
How well does Taq survive in a frost free freezer? Anthony C.
Cloning PCR products: polish ends. Brian Foley
Tnt system-in vitro translation Chris Noren
What's the L8 mutation in lac-Prom. Paul Riggs
SDS removal Paul Riggs
Does pUC18 have a T7 promoter? Tracy Aquilla
Does pUC18 have a T7 promoter? Mark Farinha
RNA oligos? Michael Powell
Isotope Incorporation? HELP ez001427 at dale.ucdavis.edu
in-vitro vs. in-vivo translation Weixing Luo
Coomassie Blue G-250 staining Unknown
Antibody clones H. David Fischer
E.coli transformation protocol wanted glover martin
High Five cells viner_n at msdisk.wustl.edu
Address needed from Syntex Research, Palo Alto! simon.lee at syntex.com
ds Sequencing Qiagen-prep Plasmids-Help! fgrun
How well does Taq survive in a frost free freezer? fgrun
5'-AmpliFINDER RACE Kit CLONTECH at BIOTECHNET.COM
High Five cells Michael Skidmore
Benchtop shaker-incubators Danuta Bois
Address needed from Syntex Research, Palo Alto! BARRY J MARGULIES
Origin of C. elegans Creg Darby
PCR questions? LEES at SASK.USASK.CA
Oligo Manufacturers Gabriel Dorado
metallothionine promoter Bulletin board login
Ultralow recommendations needed Paul A. Vescio
Quantification on phosphorimager Tracy Aquilla
sequence software Tracy Aquilla
Ultralow recommendations needed Tracy Aquilla
Altered Sites -Pros? Cons? Tracy Aquilla
Non-isotopic detection of nucleic acids Tracy Aquilla
pea genomic library needed CHRISTOPHER M. SMITH
? Swiss Protein - kw searches Tracy Aquilla
protocols on CnBr clivage of peptids Clement-Major Sebastien
Coomassie Blue G-250 staining Dietmar Tietz
sequence software Herve Recipon
Overexpression of large proteins Robert Hindges
Looking for Chicken Genome Library Nancy Shaper
genomic DNA purification questions Frederick Garbrecht
XL-1 Red---Beware! Scott Vande Pol
How well does Taq survive in a frost free freezer? Brett Beitzel
Error in Pharmacia pGEX Instructions Alan Gerstein
Oligo Cloning Seth Findley
Pfu Poly, Cloning Duncan Clark
Measuring Pi to determine ATPase:help Koller at koller
Coomassie Blue G-250 staining Marion Freistadt
protocols on CnBr clivage of peptids Alexander P Rostovtsev
Ultralow recommendations needed Sue Jeffrey
DNA isolation from low melting agarose blocks Miss C.I. Kwok
Ultralow recommendations needed Parke K. Flick
ssDNA circularization and T4 RNA ligase? Inna Vainshtein
? Swiss Protein - kw searches Tony Long
Coomassie Blue G-250 staining ez001427 at dale.ucdavis.edu
trangene newsgroup/discusion group mluo at cc.memphis.edu
Disappearing Plasmids--Help! Dennis Templeton
Peptide mapping Dennis Templeton
HA antibody 12CA5 availability? Dennis Templeton
Tnt system-in vitro translation Dennis Templeton
plasmid that won't cut Paul N Hengen
Coomassie Blue G-250 staining Paul N Hengen
avidin Graham Collins
Different types of PCR Stringer, Peta
HA antibody 12CA5 availability? Jim Woodgett
DNA isolation from low melting agarose blocks Antje Haase
How well does Taq survive in a frost free freezer? Hey leave me alone!
YAC rat library available? Allison Haggarty
Fixing sequencing gels Elisabeth Casademunt
Quantitation of small amts of DNA Shiao Y. Wang
Cracking SDS gels Mike Morris
qiagen plasmid preps Peter S. Silverstein
ligation in LMA Clemens Suter-Crazzolara
Cracking SDS gels Ashok Aiyar
PCR questions? Jim Owens
fragment isolation w/o glass milk? Compilation Daniel Schlieper
qiagen plasmid preps Stephen R. Lasky
two-hybrid cDNA libraries-fly and vertebrates? padgett at mbcl.rutgers.edu
How well does Taq survive in a frost free freezer? Anthony C.
cracking SDS PAGE brett at BORCIM.WUSTL.EDU
Cracking SDS gels Chris Larsen
PCR questions (the overhang) HARDIES at THORIN.UTHSCSA.EDU
qiagen plasmid preps Matthias Zeiner
qiagen plasmid preps B Stickney
How well does Taq survive in a frost free freezer? Zophonias O. Jonsson
gravimetric detection of oligonucs borges at almaden.ibm.com
PCR questions? Duncan Clark
frost-free freezer blues HARDIES at THORIN.UTHSCSA.EDU
Kunkel Oligo-Directed Mutagenesis Tracy Aquilla
SV-40 Promoter Bashing Joe M. SandyFaulkner, Jr.
PCR questions? Andy Law Big Nose
genomic DNA purification questions nishir at ohsu.edu
(none) Allan Gibson
Disappearing Plasmids--Help! anon
Guidelines for Tissue Culture Facility Brian T. Greuel
Overexpression of large proteins Clemens Suter-Crazzolara
Looking for a Public Domain MSDS Sheet Server Greg Carter
Disappearing Plasmids--Help! Dennis Templeton
Disappearing Plasmids--Help! contente at usuhs.usuhs.mil
RNA elution/secon. structure otello at sc2a.unige.ch
Coomassie Blue G-250 staining Elmer M. Price
Coomassie Blue G-250 staining Elmer M. Price
unsubscribe Allan Gibson
genomic DNA purification questions contente at usuhs.usuhs.mil
Qiagen/Nucleobond Cartridge Reuse Amos H Heckendorf
How well does Taq survive in a frost free freezer? Klaus.Matthaei at ANU.EDU.AU
partial library chang at GSBS18.GS.UTH.TMC.EDU
Cracking SDS gels Annette C. Hollmann
jk rob
DS DNA Sequencing Help VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
Different types of PCR Eric C. Anderson
Help needed for unstable plasmid construction Bernard Murray
SV-40 Promoter Bashing David S. Huen
Sigma Electrophoresis Unit and Gel "Smiles" MICHAEL F. FREY
genomic DNA purification questions Nicola Fidler
Thrombin cleavage problem Graham Atherton
RNAse stability Robert Slany
Coomassie Blue G-250 staining Robert Slany
Ultralow recommendations needed Paul A. Vescio
RNA elution/secon. structure user at mole.bio.cam.ac.uk
Coomassie Blue G-250 staining R.G. Walters
RNA stuck in the gel wells Grad Student Lab 2
Ultralow recommendations needed Tracy Aquilla
FISH practicioners in UK. daj (David Johnston)
Qiagen/Nucleobond Cartridge Reuse Jim Woodgett
antibodies against IL6 signaltransducing gp130 anywhere?? Horst Ibelgaufts
Thrombin cleavage problem Jim Woodgett
Taq with Triton X-100, DMSO wmelchior at fdant.nctr.fda.gov
Help needed for unstable plasmid construction szcooley at chip.ucdavis.edu
How well does Taq survive in a frost free freezer? Cornelius Krasel
ligation in LMA G Gallagher
Coomassie Blue G-250 staining Tracy Aquilla
Coomassie Blue G-250 staining Tracy Aquilla
PCR primers for mouse IFNAR? Mary Coussons-Read
sequencing kits? Darren Stauth
unstable plasmid construction HARDIES at THORIN.UTHSCSA.EDU
sequencing kits? John Brunstein
Looking for a Public Domain MSDS Sheet Server Ashok Aiyar
Re Thermal Cyclers Kevin O'Donnell
Help needed for unstable plasmid construction Stuart Kuhstoss
partial library John Nash
XL-1 Red---Beware! Brian Foley
Re Thermal Cyclers Roger Wiegand
metallothionine Lauri Lintott
mini-prep DNA isolation procedures Paul N Hengen
genomic DNA purification questions Paul N Hengen
SYBR Green I Jeanne Loring
Coomassie Blue G-250 staining Paul N Hengen
genomic DNA purification questions: protocol contente at usuhs.usuhs.mil
Pichia vectors Virginia Dress
Directional In-Gel Cloning John Watson
DEPC in RACE? Harry Menegay
List of GRAS (Generally Recognized as Safe) microorganisms Gabriel Dorado
Thrombin cleavage problem Bipin K Dalmia
genomic DNA purification questions Artur Burzynski
qiagen plasmid preps Artur Burzynski
phone # inquiry David Char
unsubscribe Parin
Server for MSDS Shaun D. Black
Competitive PCR - Questions Carl Waltenbaugh
Gel-Reading Programs Todd Giles
Matchmaker 2-Hybrid Kit Christie Doris Zerylnick
? Swiss Protein - kw searches pathology at mscf.med.upenn.edu
re-amplication of PCR product without internal primers BHWFDUNG at CPHKVX.CPHK.HK
DIG nonradioactive kit to screen cDNA library Xin-Hua Zhou
Sequencing near poly A - help Shiao Y. Wang
ATTN KGEISS / AAMRL TGF-beta expression vector wanted G Gallagher
2-D N/N Agarose Gels Anthony Smith
genomic DNA purification questions Wiggy
RNAse stability Clemens Suter-Crazzolara
pGEX GST Fusion Question... Simon Twigger
CTG as a start codon mazella
PMMA cover slips KDAVIES
Lux expression conditions?? MOUNCEY
CIP Michael G. Tencza
sequencing near poly(A) tail HARDIES at THORIN.UTHSCSA.EDU
Sequencing near poly A - help Douglas Rhoads
RNAse stability HARDIES at THORIN.UTHSCSA.EDU
E.coli transformation protocol wanted Gabriel Dorado
Potent denaturants Wolfram Brune
CTG as a start codon David Glover
qiagen plasmid preps Andy Law (Big Nose)
seq polyA mcgraw at BSCR.UGA.EDU
partial library Paul N Hengen
RNA Ali Riazi
RNA extraction Ali Riazi
Coomassie Blue G-250 staining neeraj jain
(none) lylee at BULLWINKLE.UCDAVIS.EDU
Help needed for unstable plasmid construction Klaus Salger
re-amplication of PCR product without internal primers Hong Dang
potent denaturants HARDIES at THORIN.UTHSCSA.EDU
restriction site mapping program needed gotow lisa fujiko
Amino acid grouping Shaojiu Wang
RNA Michael van Waes
restriction site mapping program needed ez001427 at dale.ucdavis.edu
QUIAGEN SPAGNOL at PLAUTO.CSATA.IT
Dyna-magnetic DNA probe for Southern Kah-Tong, Seow
CIP Peter Gegenheimer
RNAse stability Peter Gegenheimer
Fuzzy sequence gel bands Bengt Oxelman
restriction site mapping program needed Manuel G. CLAROS
E.coli transformation protocol wanted Zophonias O. Jonsson
Fuzzy sequencing gel bands Bengt Oxelman
PCR of SuperScript 1st Strand? gustilo at pobox.upenn.edu
AFP clone available? Allison Haggarty
CIP Bernard Murray
bitter sugar SPAGNOL at PLAUTO.CSATA.IT
Effects of SDS on Thermolysin? Stallion
Fixing sequencing gels CRH
Ultralow recommendations needed Curt Ashendel
regulations on transgenic plants Gary Kuleck
How well does Taq survive in a frost free freezer? Duncan Clark
Porcine liver cDNA library Kurihara Tatsuo
Current Contents online Uwe Harmening
restriction site mapping program needed Manuel G. CLAROS
Electrofusion of plant proto's via BIORAD Gene Pulser?? Jeff
PCR of SuperScript 1st Strand? Richard Near
avidin Shiao Y. Wang
Assay for biotin-PCR products Shiao Y. Wang
Making proteinase K stock Shiao Y. Wang
Porcine cDNA Library Kurihara Tatsuo
Chromosome probes for ISH Marie-Terese Little
RNase stability rhubner at molbiol.ox.ac.uk
HepG2 cells and corticosteroids Matthias Zeiner
Assay for biotin-PCR products Roger Wiegand
Current Contents online Dave Smith
erbB3 and erbB4 spiff at psycho.toppoint.de
ds Sequencing with Qiagen prep Dr. C.J. Chan
PCR primers for mouse IFNAR? (fwd) Mary Coussons-Read
SYBR Green I Scott Selby Med Heme
Fuzzy sequencing gel bands Robert Horton
Transformation problems Emma Macfarlane
Oligo that won't label Ms I G Zvetkova
Fuzzy sequencing gel bands Frosty Loechel
E.coli transformation protocol mccallo at rand
plasmid recovery from "dead" plates Kimberly Ann Winkeler
NEED: Clontech 5' RACE kit Protocol Eric C. Anderson
Use of Streptavidin coated beads Paul N Hengen
fuzzy sequencing gel bands (glycerol?) HARDIES at THORIN.UTHSCSA.EDU
bitter sugar Virginia Dress
NEED: Clontech 5' RACE kit Protocol Eric C. Anderson
Does anyone have pGEX-KN/pGEX-KT, please? Viraj Master
Spin Coluumns Peter E. Prevelige
Differential scanning calorimetry Alan Simmons
Pichia vectors Jeffrey Shane Temple
Plasmid size limitation on E. Coli Transformation? BrianC4208
Magnetic beads staff. Reusable??? Dave Knorr
Plasmid size limitation on E. Coli Transformation? Mark Farinha
Plasmid size limitation on E. Coli Transformation? elliott s. goldstein
gel sealing tape nishir at ohsu.edu
Magnetic beads staff. Reusable??? A428ENDE at VM1.SARA.NL
size limit to transformation HARDIES at THORIN.UTHSCSA.EDU
DEPC in RACE? John Watson
qiagen plasmid preps Robert Woodward
Transformation problems ez001427 at dale.ucdavis.edu
fuzzy sequencing gel bands (glycerol?) ez001427 at dale.ucdavis.edu
In situ PCR of mitochondrial DNA Jim Cummins
Chromosome probes for ISH Marie-Terese Little
TAQ contaminated with DNA? David Adelson
(none) Wafa.El-Adhami at ANU.EDU.AU
TAQ contaminated with DNA? magoldstein at ucdavis.edu
Gel sealing tapes Dr. C.J. Chan
TAQ contaminated with DNA? John Nash
Taq contamination? berschic at convex.rz.uni-duesseldorf.de
taq contamination? berschic at convex.rz.uni-duesseldorf.de
D.N.A PURIFICATION......HELP PLEASE MED6MHS at VAX.BIO.LEEDS.AC.UK
optimizing PCR reactions Frederick Garbrecht
Alternatives to 32P for Phosphorylation States? Stefan M. Gorsch
plasmid recovery from "dead" plates Kimberly Ann Winkeler
(none) jd89
need help to purify antimicrob agnts bartholo at aardvark.ucs.uoknor.edu
Plasmid size limitation on E. Coli Transformation? Paul N Hengen
TAQ contaminated with DNA? Paul N Hengen
Fixing sequencing gels. John Maris
No Subject Wolfram Brune
optimizing PCR reactions szcooley at chip.ucdavis.edu
vancomycin and Agrobacterium Bjorn Orvar
PCR inhibitors in insect tissue? edwardsg at skyfox.usask.ca
gel sealing tape Karl A. Suiter
gel sealing tape Bruce Elder
Fuzzy sequencing gel bands Dan Diaz
Excision of Bluescript from Lambda ZAP Michael Lydan
optimizing PCR reactions Mr V. Schoenfeld DOM
Alternatives to 32P for Phosphorylation States? Eric C. Anderson
subscribe Charles L. Rutherford
subscribe methods Charles L. Rutherford
unsubscribe Charles L. Rutherford
Alternatives to 32P for Phosphorylation States? Curt Ashendel
PCR optimization HARDIES at THORIN.UTHSCSA.EDU
sealing sequencing gels HARDIES at THORIN.UTHSCSA.EDU
plasmid recovery from "dead" plates Paul N Hengen
hplc column for peptide analysis Walt Lilly
E.coli transformation protocol wanted Paul N Hengen
FAQ list for bionet.molbio.methds-reagnts Paul N Hengen
Best molbio software package? Tracy Aquilla
Plasmid size limitation on E. Coli Transformation? Steve Minchin
plasmid library Jim Gale
Competitive PCR - Questions douglas l feinstein
plasmid recovery from "dead" plates Rafa Maldonado
E.coli transformation protocol Rafa Maldonado
Ortho Diagnostics address? uchmtdm at EARTH.OSCS.MONTANA.EDU
E.coli transformation protocol S. LaBonne
Help needed for unstable plasmid construction Ian A. York
E. coli PFGE Rafa Maldonado
Excision of Bluescript from Lambda ZAP John Watson
gel sealing tape John Watson
HLA-DQ typing Wachara Kasinreak
metallothionine promoter Unknown
size limit to transformation Slawek Bartoszewski
Amino acid grouping Brian Smith-White
bitter sugar Brian Smith-White
Oligo that won't label Brian Smith-White
gel sealing tape Brian Smith-White
Plasmids in the dark.. GONCZOL at WISTA.WISTAR.UPENN.EDU
Calling GERMANY: Chemie-Sicherheitsdaten Horst Ibelgaufts
Do any journals take papers on disc? Graham Atherton
D.N.A PURIFICATION......HELP PLEASE Mike Morris
E.coli transformation protocol Peter Alefounder Bioc
plasmid size limitation for transformation GWDGV1::KCHOWDH
Large scale microsatellite screening Dan Bradley
re-amplication of PCR product without internal primers douglas l feinstein
NA45 Paper DNA extraction problem Suzy Schneider
TAQ contaminated with DNA? Duncan Clark
L lactis lactis CNRZ270 Christopher Coward Path
Fixing sequencing gels. Suzy Schneider
E. coli PFGE Ted Davis
Looking for Haolin Ni Zainul Zain
Transformation problems Tracy Aquilla
ATTN: Jaz: Clontech 5' RACE Protocol Eric C. Anderson
sealing gels-shocking news HARDIES at THORIN.UTHSCSA.EDU
Differential scanning calorimetry Eric C. Anderson
plasmid recovery from "dead" plates Eric C. Anderson
selection of dimeric proteins using filamentous phage Stephane Vuilleumier
T7 RNA polymerase promoter question. Duke Groebe
Northern analysis with Renaissance Kit GUT at QUCDN.QueensU.CA
plasmid recovery from "dead" plates Eric C. Anderson
hplc column for peptide analysis Paul Donald Pyla
T7 RNA polymerase promoter question. Paul N Hengen
Competitive PCR - Questions szcooley at chip.ucdavis.edu
Best molbio software package? szcooley at chip.ucdavis.edu
large plasmids brett at BORCIM.WUSTL.EDU
mRNA storage J.P. Hays
mRNA storage Lisa J Brunet
mRNA storage Hanson Lab
fixed my plasmid problem, thanks Hanson Lab
T7 RNA polymerase promoter question. jr at dna.bio.warwick.ac.uk
gel sealing tape Norman J. Pieniazek x4073
TAQ contaminated with DNA? (fwd) Robert Preston
NA45 Paper DNA extraction problem Carlisle Landel
mRNA storage NEAL MUSTO
Manual for DNAsis-Mac V.2.0 KY HWA
Competitive PCR - Questions douglas l feinstein
D.N.A PURIFICATION......HELP PLEASE Yasha Hartberg
gel sealing tape Ashok Aiyar
biotechnology jurgenson at cobra.uni.edu
transfection of T-cells user at ichr.uwa.edu.au
mRNA storage Andre Hamel
re-amplication of PCR product without internal primers Klaus.Matthaei at ANU.EDU.AU
gravimetric detection of oligonucs Shiao Y. Wang
Mac freeware for binding? Hong Dang
<None> rosas at mcclb0.med.nyu.edu
Subcloning dirty PCR products rosas at mcclb0.med.nyu.edu
Touchdown PCR reactions Peter Kaub
Is downward transfer REALLY good for Northern? Jason Lei
Manual for DNAsis-Mac V.2.0 magoldstein at ucdavis.edu
Amplified Refractory Mutation System PCR Jim Cummins
re-amplication of PCR product without internal primers Andy Law Big Nose
site directed mutagenesis Bulletin board login
Myotube nuclei John Chesters
Insulin receptors Pinter Shlomit
TAQ contaminated with DNA? Paul N Hengen
5'-AmpliFINDER RACE Kit Paul N Hengen
Subcloning dirty PCR products Emma Macfarlane
Transformation problems Brian Foley
Dyna-magnetic DNA probe for Southern Matthew Wakefield
Library screenings by PCR raul arredondopeter
casein overlay procedure "Christine Pull ", BIO
Help! "Hot" PCR probe for Northern Blots? Jinling Xie
Two-dimensional DNA electrophoresis Richard Levenson
slow growing cultures MUNGO at UVVM.UVIC.CA
Subcloning dirty PCR products Dr. YL Du
Help! "Hot" PCR probe for Northern Blots? Mr V. Schoenfeld DOM
Fixing sequencing gels. Tracy Aquilla
FEARFUL SIMMETRY SPAGNOL at PLAUTO.CSATA.IT
RNAse inhibitor Janet E. Rubin
Library screening by PCR Emma Macfarlane
RNAse inhibitor Lisa J Brunet
Library screenings by PCR Eric C. Anderson
gel sealing tape Yasha Hartberg
Differential Display PCR/ Pulse field gel electro phoresis questions Jack M. Bernstein, M.D.
site directed mutagenesis Rafa Maldonado
Subcloning dirty PCR products Rafa Maldonado
help: phage DNA extraction pzhang at CC.UManitoba.CA
protein calorimetry Jordan Konisky
gel sealing tape nishir at ohsu.edu
Is downward transfer REALLY good for Northern? nishir at ohsu.edu
Best molbio software package? Sean David Moore
help: phage DNA extraction John Nash
affinity purification of receptor (was Re: avidin) Bryan Crawford
Subcloning dirty PCR products Steve
Teosinte library raul arredondopeter
Protein complex dissociation nievesr at TCPLINK.NREL.GOV
RNA REMOVAL: Help! ez001427 at dale.ucdavis.edu
affinity chromatography nievesr at TCPLINK.NREL.GOV
large plasmids Rafa Maldonado
Two-dimensional DNA electrophoresis Antje Haase
site directed mutagenesis ez001427 at dale.ucdavis.edu
pMEXneo sequence Toru Imamura
restriction enzyme list David N. Smith
restriction enzyme list magoldstein at ucdavis.edu
restriction enzyme list Chaisiri Wongkham
"Cold" SSCP Chris Weghorst
summary: re-amplification of PCR product using the original BHWFDUNG at CPHKVX.CPHK.HK
vancomycin and Agrobacterium Wolfram Brune
Sma I degrading ends Anthony Tomlinson
Gel-Reading Programs Anthony Tomlinson
expression vector pT7-7 or derivative Ursula Schell
E.coli transformation protocol wanted berry at biochem.unp.ac.za
Antisense oligos Dr. C.P. Healy
Oligo synthesis woes Dr. C. Dolphin
HPLC coloumn for peptides berry at biochem.unp.ac.za
RNA REMOVAL: Help! Douglas Rhoads
e.coli transformation question Andreas Weber
RNA REMOVAL: Help! Hanson Lab
site directed mutagenesis Ashok Aiyar
Antisense oligo design Dr. C.P. Healy
High MW protein standards Dave Smith
Subcloning dirty PCR products Ian A. York
fos and jun Gotz von Bulow
erbB3 and erbB4 Tracy Aquilla
vancomycin and Agrobacterium Stuart Kuhstoss
TAQ contaminated with DNA? Wolfgang Kusser
RNA REMOVAL: Help! Michael van Waes
Problem with inversed binding on a dot blot doug white
In Search of Beta Sites : (help??) MBPI at BIOTECHNET.COM
Micro Journal Club Tom Schneider
Sma I degrading ends (an alternative) HARDIES at THORIN.UTHSCSA.EDU
Problem with inversed binding on a dot blot Andreas Weber
Two-dimensional DNA electrophoresis John Brunstein
summary: re-amplification of PCR product using the original Muriel Liberto
Mwatson's 20 kbp PCR question. KASRA RAMYAR
Do any journals take papers on disc? Ian A. York
To Georges from France, Hong Dang
optimizing PCR reactions Ron Kagan
subcloning dirty pcr products Emma Macfarlane
RNA REMOVAL: Help! ez001427 at dale.ucdavis.edu
avidin T. S. Pillay
Insulin receptors T. S. Pillay
Alternatives to 32P for Phosphorylation States? T. S. Pillay
Best molbio software package? T. S. Pillay
restriction enzyme list
Sequencing Gel Spacer Plastic Anthony Otsuka
mRNA storage Peter Gegenheimer
T7 RNA polymerase promoter question. Peter Gegenheimer
Best molbio software package? Kevin Morano
Do any journals take papers on disc? Kevin Morano
Cloning in Afl III site in pUC vectors Duncan Clark
Lost DNA after precipitation Duncan Clark
Afl III site in pUC vectors Duncan Clark
Sequence or Rest. Map for pGP1-2 Duncan Clark
Growing Up TA Cloning Products Andrew McArthur
Differential Display PCR Michael Joiner
mRNA storage Seth Corey
Hints for low-stringency cloning Tae Bum Shin
cDNA library kits, which is best? Richard Dana
hplc column for peptide analysis Azlinda Bte Anwar
TAQ contaminated with DNA? Duncan Clark
Afl III site in pUC vectors Duncan Clark
Cloning in Afl III site in pUC vectors Duncan Clark
ß-galactosidase and NP-40 Karl Fischer
Growing Up TA Cloning Products Lisa J Brunet
Growing Up TA Cloning Products douglas l feinstein
cDNA library kits, which is best? douglas l feinstein
Why PCR reamp = smears. HARDIES at THORIN.UTHSCSA.EDU
in vitro mutagenesis David Adelson
Promoter and DNA consensus-finding software Ron Kagan
HPLC column for peptides MAHER DERBEL
NORTHERN BLOTS Philip OBrien
dihydordiol purchase ?? Lee
Best molbio software package? T. S. Pillay
Protein electroelution from SDS-page T. S. Pillay
High MW protein standards T. S. Pillay
Growing up TA cloning products Graham Atherton
pseudogene Dr. B. Sylla
Detection of H3 expression (fwd) vetfisio at IPRUNIV.CCE.UNIPR.IT
Hints for low-stringency cloning Gill Diamond
PCR from Fibroblasts? Scott Wildenberg DGM-IHG
tRNA nucleotidyl transferase Jitao
PCR from Fibroblasts? Mike Morris
Detecting mutations. Dr. G.R. Taylor
Densitometry software? Mr TG Downing
Subcloning dirty PCR products Tomas Bratt
subcloning dirty pcr products Torben Osterlund
Subcloning dirty PCR products schupp at nauvax.ucc.nau.edu
vancomycin and Agrobacterium szcooley at chip.ucdavis.edu
Hints for low-stringency cloning John Watson
ß-galactosidase and NP-40 schupp at nauvax.ucc.nau.edu
mRNA storage pgegen at RNAWORLD.BIO.UKANS.EDU
mRNA storage John Watson
Densitometry software? schupp at nauvax.ucc.nau.edu
glycosylated protein isolation jgill at kean.ucs.mun.ca
Why PCR reamp = smears. (fwd) Robert Preston
tRNA nucleotidyl transferase Jitao
Poly A RNA Grant McKegney
affinity purification of receptor (was Re: avidin) Cornelius Krasel
HELP with sequencing acc NAR 16(22):10915 David Johnson
HELP with sequencing acc NAR 16(22):10915 David Johnson
RNAse inhibitor B Stickney
Qiagen/Nucleobond Cartridge Reuse Paul N Hengen
anthocyanins and protein isolation Nokwanda Makunga
RNA isolation from small # of cells? bormann at urz.unibas.ch
Cloning Repetitive DNA WATSONC at QUCDN.QueensU.CA
plasmid recovery from "dead" plates Paul N Hengen
transfection of T-cells Mike Klopfer
TAQ contaminated with DNA? Paul N Hengen
PCR from intact bacterial cells mohanish at pharmdec.wustl.edu
Why PCR reamp = smears. HARDIES at THORIN.UTHSCSA.EDU
Why PCR reamp = smears. (fwd) Klaus.Matthaei at ANU.EDU.AU
Hints for low-stringency cloning Doc Martin
Densitometry software? Robert Horton
hIL-4Ralpha primers: suggestions please davis at wehi.edu.au
PCR from intact bacterial cells Marc A. Goldstein
direct sequencing from chromosome? adler at staff.monash.edu.au
anthocyanins and protein isolation William.Cress
S:avrI and sciI; recognition&cut? Professor Rudolf Prof. Dr. Eichenlaub
Nonisotopic AA transport assay? Hr Dr. S. Shapiro
Growing Up TA Cloning Products Duncan Clark
RNA from E.coli Bulletin board login
Medical Genetics management s/ware Mike Morris
temp.sensitive ori / pBR322 deriv. FJ van der Wal
RNA REMOVAL: Help! Jim Owens
General note on distribution of vectors strains etc. Duncan Clark
Microwave Miniprep - protocol? Robert Rumpf
Buying Hyb. Oven/Roller ktgeiss at miavx1.acs.muohio.edu
e.coli transformation question Duke Groebe
PCR inhibitors in insect tissue? Tracy Aquilla
E. coli PFGE Tracy Aquilla
mRNA storage Tracy Aquilla
large plasmids Tracy Aquilla
fos and jun Tracy Aquilla
Biotin in hybridisation. Dr. A. Rosenthal
membrane preparation from COS cell Georges Gaudriault
Why PCR reamp = smears. (fwd) szcooley at chip.ucdavis.edu
DNA from lypholized tissue? szcooley at chip.ucdavis.edu
PGEM accession numbers Tod Dwayne Strugnell
pGEM accession numbers Tod Dwayne Strugnell
Growing Up TA Cloning Products Chong-Yee Khoo
Plasmid size limitation Cynthia Shippam
Gel sealing tapes Cynthia Shippam
Gel sealing tapes Cynthia Shippam
S:avrI and sciI; recognition&cut? Mark Farinha
Rapp Polymere resin smithr at MRC.COM
Buying Hyb. Oven/Roller lehn at facstaff.wisc.edu
e.coli transformation question Rafa Maldonado
in vitro mutagenesis Yasha Hartberg
Where can I get DNA Strider 1.2? Tracy Aquilla
Growing Up TA Cloning Products Carlisle Landel
Buying Hyb. Oven/Roller Mark Farinha
rescue dead stock elliott s. goldstein
anthocyanins and protein isolation Peter Gegenheimer
T7 Expression System Klara Briknarova
Primers with dI vs mixed bases? Allen Gathman
PCR from intact bacterial cells Robert Hindges
HBSAG Salvatore Sechi
Buying Hyb. Oven/Roller James S. Sutcliffe
PI values Richa Behari
Protein electroelution from SDS-page Roland J Saldanha
wheat library for 2-hybrid system Richard Dana
transfection of T-cells Jonathan M Kaplan
Looking for mol bio software.... Frank White
RNA REMOVAL: Help! Dongxian Yue
PNK substrates Dongxian Yue
Any experience with Soccorex pipettors Simon Ginsberg
Ultralow freezer - summary Shiao Y. Wang
Where can I get DNA Strider 1.2? Pierre Martineau
Socorex pipettes Mike Morris
(none) 126DANIE at WITSVMA.WITS.AC.ZA
(none) Gotz von Bulow
Any experience with Soccorex pipettors daj (David Johnston)
RNA from E.coli Cornelius Krasel
where is Cambridge Res. Biochem.? smithr at MRC.COM
Sequencing using infrared tags Ann Kleinschmidt
Problem getting JM110 competent Tom
where is Cambridge Res. Biochem.? Dr R. Dalgleish
How to treat cells in interphase stage for FISH? Wasun Chantratita
long range PCR m-brazil at nimr.mrc.ac.uk
T7 Expression System Bernard Heymann
Best molbio software package? Yasha Hartberg
PCR from intact bacterial cells Julian Dow
mRNA storage B Stickney
lambda/HindIII B Stickney
protein elution Tzahi Arazi
Oddity on sequencing gels Bruce Elder
where to get Rapp Polymere TentaGel? smithr at MRC.COM
plasmid drawing shareware? gr8bn at cc.usu.edu
SYBR Green I, more experiences Jun Kim
info about cosmids Jeffrey D. Bray
Inexpensive blotting paper? Dennis Templeton
SYBR Green I, again Jun Kim
PCR with 3' mismatch HARDIES at THORIN.UTHSCSA.EDU
RC20 anti-phosphotyrosine Ab: non-specific detection William R. Morgan
vancomycin and Agrobacterium szcooley at chip.ucdavis.edu
rescue dead stock Rachelle Leger
protein elution Klaus.Matthaei at ANU.EDU.AU
PCR from intact bacterial cells Rachelle Leger
pGEM accession numbers ez001427 at dale.ucdavis.edu
T7 Expression System ez001427 at dale.ucdavis.edu
Taq DNA Pol and frameshifts Michael J Marino
Oddity on sequencing gels ez001427 at dale.ucdavis.edu
lambda/HindIII Andrew Cockburn
T7 Expression System Richard T. Timmer
ABI Sequencer Facility management? We've recently set up an ABI 373 sequencer in a shared facility. Any net suggestions as to software to simplify the MANAGEMENT of the facility (i.e., Excel spreadsheet templates for sample entry, automated mailing of users results, etc,,,)? Seems like this issue may have been dealt with before. I've already heard of Toolkit... Thanks in advance for any info, Richard R. Hardy
RE- Soccorex pipettors Dieter Palmer

Last message date: Wed Aug 31 22:44:08 EST 1994
Archived on: Sun Mar 6 20:10:47 EST 2005

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