Methods August 1994 Archives by thread

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Starting: Mon Aug 1 03:48:24 EST 1994
Ending: Wed Aug 31 22:44:08 EST 1994
Messages: 1080

"sticky" sequencing gels jenkins at aidsun.nibsc.ac.uk
antibody production Clemens Suter-Crazzolara
- antibody production Jello ease
- antibody production Peter J. Crilly
NM1149 cloning site sequence? Frances Hannan Zoology
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
Sequence or Rest. Map for pGP1-2 Christopher Walentas Bioc
- Sequence or Rest. Map for pGP1-2 Robert Solomon Bioc
- Sequence or Rest. Map for pGP1-2 Duncan Clark
Advice on ABI373A Sequencing Conditions? Bruce Roe
Help needed about Branson Sonifier! Inst. f. Biochemie
Non EtBr DNA stain(s) Andrew Cockburn
Universal PCR Dick Beeman
Gel Purification of DNA with Qiaex beads Clemens Peterbauer
EMSA-Competition problem Peter Brunovskis
Ni/His-tag 4 affinity purification of ABs? Anne Spang
PCR and Strand Displacement Parke K. Flick
Dissuruption Buffer szcooley at chip.ucdavis.edu
More GelReader Manuel G. CLAROS
Pointer to Biologist's Internet Resource Guide Requested Susan Kretschmer
- Pointer to Biologist's Internet Resource Guide Requested Christopher B. Yohn
  - Pointer to Biologist's Internet Resource Guide Requested Christopher B. Yohn
  - Biologist's Guide IS on WWW Una Smith
BTX electroporators--info please? ALLEN, BEVERLY S
sequence software Seth Daniel Crosby
- sequence software Gill Diamond
  - sequence software Tracy Aquilla
    - sequence software Herve Recipon
- sequence software Robert Woodward
Lost DNA after precipitation Zengjian Chen
- Lost DNA after precipitation Pendragon
- Lost DNA after precipitation schupp at nauvax.ucc.nau.edu
  - Lost DNA after precipitation Annette C. Hollmann
- Lost DNA after precipitation Duncan Clark
PCR woes Purnima Laud
- PCR woes schupp at nauvax.ucc.nau.edu
- PCR woes Satyam Subrahmanyam
  - More GelReader Anthony Tomlinson
  - More GelReader Manuel G. CLAROS
mammalian expression vector Seth Daniel Crosby
EMSA-Competition problem gc
Oligo degradation trepidation szcooley at chip.ucdavis.edu
Qiaex problems (was Re: Gel Purification of DNA with Qiaex beads) neale at mbcf.stjude.org
- Gel Purification of DNA with Qiaex beads Anthony Tomlinson
  - Gel Purification of DNA with Qiaex beads neale at mbcf.stjude.org
Oligo degradation trepidation Thomas M. Rupp
***PLEASE HELP with NADH*** X-Man
In situ hybridization of cell suspensions? Bohdana Badzio
Custom baculoviral expression services Garry Myers
- Custom baculoviral expression services Steve Rodems
Dissuruption Buffer daj (David Johnston)
- Dissuruption Buffer dpetersn at unixg.ubc.ca
Transformation method for Rhodobacter capsulatus? Mareike Trieschmann
Kit for sequencing mRNA Henrik Wengholt
Non EtBr DNA stain(s) Sebastian W. Bunka
Anyone have a spare cell disruptor? Brian T. Greuel
RNA isolation Brett Beitzel
mammalian expression vector pskaytes at INTNET.UPJ.COM
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
Reused Electroporation Cuvettes? George W Chacko
- Reused Electroporation Cuvettes? Dick Vogt
- Reused Electroporation Cuvettes? szcooley at chip.ucdavis.edu
  - Reused Electroporation Cuvettes?
- Reused Electroporation Cuvettes? Nancy Lemieux
  - Reused Electroporation Cuvettes? Jon Visick
    - Reused Electroporation Cuvettes? eric c. anderson
mammalian expression vector Clemens Suter-Crazzolara
Modified dNTPs Gerri Newfry
help with in-situ hyb. zamir at agri.huji.ac.il
Universal PCR Doug Rhoads
- Universal PCR Dick Beeman
Lipid Peroxidation Stains for EM histology Gary Krause
pI of viral protein AKEY HUNG
Silver staining of DNA (help) Larry Hale
- Silver staining of DNA Mike Morris
- Silver staining of DNA dkim at unm.edu
- Silver staining of DNA (help) micha at myhost.subdomain.domain
Biologist's Internet Resource Guide Emma Macfarlane
- Biologist's Internet Resource Guide Una Smith
HELP!!! PCR Smear! Brian Foley
Fixing sequencing gels Kilroy, Gail - Dr. York 2545
Advice on ABI373A Sequencing Conditions? Steve Marsh
Monocyte blues: help! tronni at ktl.fi
- Monocyte blues: help! Ben Vowels
Need RNA Isolation Protocol Jacqueline J Janka
SURAMINE Vuk Stambolic
Chromosome probes for ISH Marie-Terese Little
subscribe Eduardo A. Garcia-Zepeda 7356000
RNA labeling with pCp and ligase ? Frank Ma
half-life of mRNA in bacteria (specifically Psuedomonas aeruginosa) hallinanp at topaz.cqu.edu.au
DNA Fingerprinting U27111 at uicvm.uic.edu
- DNA Fingerprinting Orange County Sheriff Forensic Science
  - DNA Fingerprinting U27111 at uicvm.uic.edu
    - DNA Fingerprinting Barbara Greene
    - DNA Fingerprinting Dana Macelis-Roberts Lab
    - DNA Fingerprinting Jason Fawcett
Baculovirus Jane Harrison
- Baculovirus Sean David Moore
- Baculovirus Steve Rodems
- Baculovirus Tracy Aquilla
HELP!!! PCR smears! Bengt Oxelman
in vitro transcription question Paul Thompson
- in vitro transcription question Virginia Dress
- in vitro transcription question Aaron Pawlyk
- in vitro transcription question Tracy Aquilla
About 32PdCTP conc. in quantitative PCR 石塚俊治
'Leaky' IPTG inducible promoters... Simon Twigger
- 'Leaky' IPTG inducible promoters... John McDougall
- 'Leaky' IPTG inducible promoters... Duncan Clark
- 'Leaky' IPTG inducible promoters... Tracy Aquilla
pulse-chase w/T7 system Julie Forney
Variability Between Plasmid Preps in Transfections Yun-Long Du
leaky IPTG inducible promoters Emma Macfarlane
RNA gels: Autoclaving MOPS buffer? herzer at urz.unibas.ch
- RNA gels: Autoclaving MOPS buffer? Too Heng-Phon
particle-gun eric c. anderson
RNA gels: Autoclaving MOPS buffer? Carlisle Landel
'Leaky' IPTG inducible promoters... Bernard Heymann
Split up methods-reagents? Bert Popping
genbank submission Marion Freistadt
- genbank submission John Watson
- genbank submission Paul Riggs
- genbank submission Diana Airozo
recycle GST-agarose beads? shiha02 at mcclb0.med.nyu.edu
Perfusing a mouse John Watson
Kan vs Amp for eukaryotic vectors ? John Watson
Reference Manager vs Endnote John Watson
Variability Between Plasmid Preps in Transfections Brian Foley
Alpha-1-pi Elisa protocol wanted kathy frees
summary of mycoplama detect Patrick Grealish
RT PCR-any suggestions? Tracy Aquilla
- RT PCR-any suggestions? Too Heng-Phon
- RT PCR-any suggestions? Annette C. Hollmann
- RT PCR-any suggestions? Hanson Lab
Streptavidin-coated microtiter plates walling at wmvx.lvs.dupont.com
PFGE Southern Blots Panhead McNipper
particle-gun walling at wmvx.lvs.dupont.com
in vitro transcription question Bohdana Badzio
silver staining of DNA Allergan
UV-transparent microtiter plates John H McDonald
Large-scale DNA cleanup szcooley at chip.ucdavis.edu
Glass Matrix MBPI at BIOTECHNET.COM
RNA gels: Autoclaving MOPS buffer? David Glover
split up/sequencing gels Patricia Foster
- split up/sequencing gels Zophonias Jonsson
Ecdysone receptor William W.-G. Jia
RNA gels: Autoclaving MOPS buffer? Aaron Pawlyk
PFGE Southern Blots Antje Haase
RNA gels: Autoclaving MOPS buffer? Kelly Tetro
- RNA gels: Autoclaving MOPS buffer? Tom Thatcher
- RNA gels: Autoclaving MOPS buffer? Tracy Aquilla
  - RNA gels: Autoclaving MOPS buffer? neeraj jain
    - RNA gels: Autoclaving MOPS buffer? Jim Owens
CIPPers for breakfast Pendragon
- CIPPers for breakfast Carlisle Landel
Plasmid Prep Scale-up Pendragon
Split up methods-reagents? Pendragon
- Split up methods-reagents? Bernard Murray
  - Split up methods-reagents? Anne Spang
- Split up methods-reagents? m.c. evers
Fixing sequencing gels Pendragon
High level protein expression MMKania
HELP! How to kill phages? Troels Wind
Cloning into Afl III site in pUC based plasmids Duncan Clark
- Cloning into Afl III site in pUC based plasmids Ward Lab
UK distributor for Branson Sonicators Duncan Clark
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
PAGE gels cracking Jamie Cope
RNA gels oesch at hifo.unizh.ch
Autoclaving MOPS buffer Dr. C.J. Chan
Amersham site mutagensis kit? Yi Dou
rat random cDNA ??? K.D.Preuss
Transformation advice needed? Me too! Stefan Kahlert
- Transformation advice needed? Me too! Sue Jeffrey
- Transformation advice needed? Me too! Stuart Kuhstoss
- Transformation advice needed? Me too! Song Tan
Ultralow recommendations needed Shiao Y. Wang
- Ultralow recommendations needed szcooley at chip.ucdavis.edu
  - Ultralow recommendations needed Bert Popping
    - Ultralow recommendations needed Tracy Aquilla
    - Ultralow recommendations needed Paul A. Vescio
    - Ultralow recommendations needed Sue Jeffrey
    - Ultralow recommendations needed Paul A. Vescio
    - Ultralow recommendations needed Tracy Aquilla
- Ultralow recommendations needed Lisa J Brunet
- Ultralow recommendations needed schupp at nauvax.ucc.nau.edu
DNA Fingerprinting Dave
Agarose-PAGE Gels Scott Selby Med Heme
Silver Staining of DNA Allergan
Detergent Quantification fintan_v at Msu.oscs.montana.edu
Help? Silver Stain DNA sequencing. Yi Dou
metal probe BTC-5N ez041225 at chip.ucdavis.edu
mammalian expression vector SYE at OPUS.MCO.EDU
Protein Phosphatase 1 or 2A cDNA clone LONG,Benedict
- Protein Phosphatase 1 or 2A cDNA clone John Watson
Wanted: Ferredoxin Assay dmncli02 at uctvax.uct.ac.za
coli RNA polymerase F.J.van der Wal
Dissuruption Buffer daj (David Johnston)
mRNA quantitation with the ABI 373A/GeneScan Gabriel Dorado
DNA fingerprinting with ABI 373A DNA Sequencer/GeneScan Gabriel Dorado
german whatman 3MM equivalent Clemens Suter-Crazzolara
- german whatman 3MM equivalent Robert Slany
373A Automated Sequencer etc... ir (Ian Ridgers)
glycogen in sequencing rxns??? russos at HAL.HAHNEMANN.EDU
unsubscrib HERBERT WYCKOFF
unsubscribe HERBERT WYCKOFF
DNA (oligo manufacturers) Joe M. SandyFaulkner, Jr.
- DNA (oligo manufacturers) Robert Horton
- DNA (oligo manufacturers) Tracy Aquilla
E Mal il and methods and reagents MBPI at BIOTECHNET.COM
Cycle sequencing of PCR products Tony Kwan
- Cycle sequencing of PCR products hpotter at chmeds.ac.nz
- Cycle sequencing of PCR products schupp at nauvax.ucc.nau.edu
  - Cycle sequencing of PCR products Mr V. Schoenfeld DOM
Kunkel Oligo-Directed Mutagenesis Chris Greene
- Kunkel Oligo-Directed Mutagenesis Iain Wilson
  - Kunkel Oligo-Directed Mutagenesis Tracy Aquilla
373A Automated Sequencer etc... eric c. anderson
Sequencing from boiling mini-preps? Jean Valjean
- Sequencing from boiling mini-preps? Bruce Roe
- Sequencing from boiling mini-preps? John Nash
- Sequencing from boiling mini-preps? Greg Gloor
- Sequencing from boiling mini-preps? Tracy Aquilla
Tips for 12 chan. multi-pipette David MacHugh
Silver Staining of Sequencing Gels Allergan
373A Automated Sequencer etc... Dave Dudley
- 373A Automated Sequencer etc... Mr V. Schoenfeld DOM
reusable sponges for southerns stewart at camis.stanford.edu
glycogen in sequencing rxns??? Domingos henrique
_E. coli_ 13706???? Jamie Stanek
- _E. coli_ 13706???? Duncan Clark
RNA gels Dr. G. Koraimann
Silver Stained Sequencing Gels and EDF Film Development Allergan
SYMBR Green 1 carlos h. pedemonte
- SYBR Green 1 Michael van Waes
Ligand Pharm. email address? Doc Martin
HELP! Need to make ultra-complex plasmid library Ben-Sapiens
BTC-5N metal probe (re-post) ez041225 at chip.ucdavis.edu
- BTC-5N metal probe (re-post) Raffaele Matteoni
Scanning Cell Culture Colonies Kim Konrad
- Scanning Cell Culture Colonies TJR
** Help with AO staining of Drosophila salivary glands ** Sinisac Mike
Gel Purification of DNA with Qiaex beads Marion Freistadt
PCR detection Ian Dunn
Best Way to Sequence PCR Products? gustilo at pobox.upenn.edu
- Best Way to Sequence PCR Products? Mr V. Schoenfeld DOM
Biotin and BCA protein assay! Prachya Kongtawelert
Protocol for Fluorescently Labeling Antibody Wanted Albert Smith
Osmotic Lysis of Erythroid cells - help wanted! Mr. A.F. Hill
- Osmotic Lysis of Erythroid cells - help wanted! DjayR
Journal: Molecular & Cellular Probes - anyone have Email address? Peter M. Muriana
Cycle sequencing of PCR products Frank White
Best Way to Sequence PCR Products? Frank White
Protocol for Fluorescently Labeling Antibody Wanted Flip Hoedemaeker, CvF RUL/TNO, Leiden, The Netherlands
<None> sstrain_ssb at opal.tufts.edu
PC-12 sub-clones needed sstrain_ssb at opal.tufts.edu
Quantification on phosphorimager Elizabeth Rosenberg
Quantification on phosphorimager Elizabeth Rosenberg
- Quantification on phosphorimager Tracy Aquilla
SUMMARY: Help on DNA quantification and RAPD... Naresh C. Pancholi
SUMMARY-2: Help on DNA quantification and RAPD... Naresh C. Pancholi
Two-subunit luciferase in yeast expression vector? Martin Latterich
help on LacZ needed... tao at mbcf.stjude.org
- help on LacZ needed... Paul Riggs
PNGaseF SPAGNOL at PLAUTO.CSATA.IT
microsatellites in seattle Beth Karen Wapelhorst
codon usage Database? Dave Pilgrin
Wanted, Rabbit Muscle cDNA library!! Peter Kristensen
codon usage Database? Peter Weber
- codon usage Database? Michael Coyne
- codon usage Database? Tim Buss
RNA extraction from plants? SUSI
fragment isolation w/o glass milk? Daniel Schlieper
- fragment isolation w/o glass milk? John Brunstein
- fragment isolation w/o glass milk? nishir at ohsu.edu
  - fragment isolation w/o glass milk? Sean David Moore
disappearing bands Frederick Garbrecht
- disappearing bands Aaron Pawlyk
simple method for sequencing from boiling mini-prep Chuck Miller
Gel Shift Assay--Probe in Wells Peter Brunovskis
- Gel Shift Assay--Probe in Wells gc
  - Gel Shift Assay--Probe in Wells Michael Robert Ladomery
- Gel Shift Assay--Probe in Wells Steve Rodems
Non-fixing and non-drying sequencing gel Ho-Hyung Woo
E.coli strains with no lacZ, anyone have such a beast? tao at mbcf.stjude.org
- E.coli strains with no lacZ, anyone have such a beast? Mark Farinha
Degenerate PCR Sean David Moore
- Degenerate PCR Yasha Hartberg
subscribe Allergan
E.coli transformation protocol wanted ez003450 at chip.ucdavis.edu
- E.coli transformation protocol wanted Gabriel Dorado
  - E.coli transformation protocol wanted glover martin
    - E.coli transformation protocol wanted Gabriel Dorado
    - E.coli transformation protocol wanted Zophonias O. Jonsson
    - E.coli transformation protocol wanted Paul N Hengen
Carbonate Extraction Rizwan Haq
Cycle sequencing of PCR products (fwd) Robert Preston
- Cycle sequencing of PCR products (fwd) Mr V. Schoenfeld DOM
Altered Sites -Pros? Cons? jcuozzo at opal.tufts.edu
- Altered Sites -Pros? Cons? douglas gray cancer research
- Altered Sites -Pros? Cons? Simon E Plyte
- Altered Sites -Pros? Cons? Tracy Aquilla
Licensed Taq prices Duncan Clark
- Licensed Taq prices Paul A. Vescio
Sequencing from boiling mini-preps? berry at biochem.unp.ac.za
Automated sequencing Graham Atherton
- Automated sequencing John E. Fox
- Automated sequencing eric c. anderson
Plant Nucleic Acid Isolation Richard Dana
Reference Manager vs Endnote Ola Myklebost
cellulose acetate gel electrophoresis AMRUT S BHOGLE
TRUE OR FALSE? SPAGNOL at PLAUTO.CSATA.IT
- TRUE OR FALSE? David L. Haviland, Ph.D.
Slot-Blot quantification of abundant mRNAs Allison Hanly
E.coli strains with no lacZ, anyone have such a beast? szcooley at chip.ucdavis.edu
DNA extraction from pollen szcooley at chip.ucdavis.edu
c-src T. S. Pillay
PCR thermal cyclers. Peter J. Crilly
- PCR thermal cyclers. Daniel P. Kolk
- PCR thermal cyclers. Andy Law Big Nose
9-cis retinoic acid sources needed Gary L. McKnight
Fixing sequencing gels cdickel at eye1.eye.ufl.edu
- Fixing sequencing gels Nicola Fidler
- Fixing sequencing gels Yasha Hartberg
  - Fixing sequencing gels CRH
- Fixing sequencing gels Elisabeth Casademunt
test frandenm at TCPLINK.NREL.GOV
Non-isotopic detection of nucleic acids Peter Leif Bergsagel
- Non-isotopic detection of nucleic acids Klaus Salger
- Non-isotopic detection of nucleic acids Scott Selby Med Heme
- Non-isotopic detection of nucleic acids walling at wmvx.lvs.dupont.com
- Non-isotopic detection of nucleic acids Tracy Aquilla
muscle protein extracts Jodie Mangor
non-radioactive detection? AARON J. MOE
- non-radioactive detection? walling at wmvx.lvs.dupont.com
terminal transferase & PCR, Brian Foley are you listening? Zophonias O. Jonsson
- terminal transferase & PCR, Brian Foley are you listening? Brian Foley
ERBB2 Muriel Liberto
non-radioactive detection? John Nash
Poly dT cellulose nika
NIH Image to read sequencing gels? Craig E. Cameron
non-radioactive detection? Andre Hamel
non-radioactive detection? Andre Hamel
Lambda gt library D.J. WISE
non-radioactive detection? John Nash
Licensed Taq prices Frank White
need help in analysing fatty acids rgroup at aardvark.ucs.uoknor.edu
Best way to size select cDNA ? Dave Smith
- Best way to size select cDNA ? Thomas H. Rude
SDS removal agmclen at liv.ac.uk
- SDS removal Timothy Bushnell
- SDS removal Paul Riggs
Long Ranger sequencing gels Bengt Oxelman
- Long Ranger sequencing gels Paul A. Vescio
- Long Ranger sequencing gels Mark Farinha
  - Long Ranger sequencing gels Brandon S Johnson
Common Forward Primers Ruari MacDonald
Azure C nucleus stain Yoshiki Kawabe
stripping Western blots Peggy Halleck
- stripping Western blots FJ van der Wal
- stripping Western blots walling at wmvx.lvs.dupont.com
Methods for RNA purification? Hubert Forkl
DNA (oligo manufacturer Margaret Shirra
inquiry: base-pair stability constants Erik Forbes Y. Hom
- inquiry: base-pair stability constants Greg Gloor
Autoclaving MOPS buffer/TRIS buffer dkim at unm.edu
Bact. luciferase reporter vectors schupp at nauvax.ucc.nau.edu
Maize cDNA Library Wanted enyedi at wmich.edu
To Help or Not To Help Steve Konings
- To Help or Not To Help Yasha Hartberg
Gelatin as blocking agent in Westerns Peter Heifetz
- Gelatin as blocking agent in Westerns awb at aber.ac.uk
- Gelatin as blocking agent in Westerns Andre Hamel
- Gelatin as blocking agent in Westerns Shahram Mori
Quantitation of small amts of DNA Richard R. Hardy
- Quantitation of small amts of DNA Nicola Fidler
- Quantitation of small amts of DNA John Watson
- Quantitation of small amts of DNA Anita Gould
  - Quantitation of small amts of DNA Shiao Y. Wang
one-step RNA isolation reagents Amanda Hayward-Lester
DNA (oligo manufacturer Jack M. Bernstein, M.D.
help: leaky expression vector? Knaebel David B
- help: leaky expression vector? FJ van der Wal
To Help or Not To Help Ron Kagan
spectinomycin amplification of plasmid Joe M. SandyFaulkner, Jr.
Shoddy Ann Arbor Plastics Products. Stephen R. Lasky
- Shoddy Ann Arbor Plastics Products. Chris Noren
  - Shoddy Ann Arbor Plastics Products. Stephen R. Lasky
Shoddy Ann Arbor Plastics Products. Ron Kagan
Illigitimate/Ectopic Transcription Carla Drebing
Nucleic Acid Purification MBPI at BIOTECHNET.COM
Non EtBr DNA stain(s) Paul N Hengen
High Five cells Robert C. Colgrove
- High Five cells Cornelius Krasel
  - High Five cells dallas at mcvax4.d48.lilly.com
    - High Five cells Harry Menegay
    - High Five cells Michael Skidmore
- High Five cells viner_n at msdisk.wustl.edu
stripping Western blots Scott Selby Med Heme
Oligo Cloning Ben-Sapiens
Inoue Transformation Method Ben-Sapiens
Mwatson's 20 kbp PCR question. Tracy Aquilla
- Mwatson's 20 kbp PCR question. KASRA RAMYAR
NA45 Paper DNA extraction problem Tracy Aquilla
- NA45 Paper DNA extraction problem Suzy Schneider
  - NA45 Paper DNA extraction problem Carlisle Landel
F' restriction sites Patricia Foster
Sma chewing back - suggestions? Dr S T May
373A-stretch survey hollingswor at immunex.com
- 373A-stretch survey Steve Marsh
** Help w/ AO staining of drosophila salivary glands Sinisac Mike
[Q]cycle sequencing Toshio Ikeda
Strand Displacement Amplification Duncan Clark
- Strand Displacement Amplification Dick Beeman
3 PRIME AND 5 PRIME RACE JŸrgen Wendland
ligation not working? Tomas Bratt
- ligation not working? Brian Foley
DNA from Pollen LONSDALE
plasmid that won't cut Hanson Lab
- plasmid that won't cut Paul N Hengen
Annealing Temperatures for PCR rosas at mcclb0.med.nyu.edu
- Annealing Temperatures for PCR Paul A. Vescio
- Annealing Temperatures for PCR schupp at nauvax.ucc.nau.edu
- Annealing Temperatures for PCR Brian Foley
Modified dNTPs ott at wmvx.lvs.dupont.com
Sequencing plates Ian Wood,SR306,3632
- Sequencing plates glover martin
- Sequencing plates Tanya Osickey
one-step RNA isolation reagents contente at usuhs.usuhs.mil
What's the L8 mutation in lac-Prom. Frank Buchholz
- What's the L8 mutation in lac-Prom. Paul Riggs
Gelatin as blocking agent in Westerns frandenm at TCPLINK.NREL.GOV
Annealing Temperatures for PCR fgrun
3 PRIME AND 5 PRIME RACE John Watson
Annealing Temperatures for PCR John Watson
Annealing Temperatures for PCR Clemens Suter-Crazzolara
Radioisotope Joe M. SandyFaulkner, Jr.
sma I chewing back - any suggestions? txpljfg at UABCVSR.CVSR.UAB.EDU
The roles of gelatin, tween20 and glycerol in PCR Alex Chang
Oligo Manufacturers Joe M. SandyFaulkner, Jr.
- Oligo Manufacturers schupp at nauvax.ucc.nau.edu
  - Oligo Manufacturers Shahram Mori
  - Oligo Manufacturers Gabriel Dorado
Semi dry blotting caution! Dennis Templeton
EIA software? 93huebner at wmich.edu
- EIA software? Sorin Damien
Digene Fastpair B Stickney
Sma I degrading ends HARDIES at THORIN.UTHSCSA.EDU
- Sma I degrading ends Anthony Tomlinson
chloroplasts & rho factor? Bob Gross
- chloroplasts & rho factor? Bob Gross
Gel Purification of DNA frandenm at TCPLINK.NREL.GOV
test Stringer, Peta
Tnt system-in vitro translation T. S. Pillay
- Tnt system-in vitro translation Steve Rodems
- Tnt system-in vitro translation Chris Noren
  - Tnt system-in vitro translation Dennis Templeton
Qiagen Plasmid Preps kalmar at sfu.ca
drug selection on CHO cells David Char
HSV-1 VP16 antibody? PETER PECHAN
? Swiss Protein - kw searches Tony Long
- ? Swiss Protein - kw searches Tracy Aquilla
  - ? Swiss Protein - kw searches Tony Long
    - ? Swiss Protein - kw searches pathology at mscf.med.upenn.edu
Removing ssDNA from dsDNA - help Joe DeRisi
sequencing kits? David M Grieger
- sequencing kits? Darren Stauth
ELISA for TRH Robert Bateman
Removing ssDNA from dsDNA - help Scott B. Mulrooney
Origin of C. elegans Rafa Maldonado
- Origin of C. elegans Stephen Nurrish
  - Origin of C. elegans eric c. anderson
  - Origin of C. elegans Creg Darby
Address needed from Syntex Research, Palo Alto! Inst. f. Biochemistry
- Address needed from Syntex Research, Palo Alto! simon.lee at syntex.com
- Address needed from Syntex Research, Palo Alto! BARRY J MARGULIES
Address from Syntex Research, Palo Alto needed! Inst. f. Biochemistry
erbB2 gene Muriel Liberto
Address from Syntex Research, Palo Alto needed! Robin Ann Wasserman
ELISA for TRH Robert Bateman
plasmid that won't cut HARDIES at THORIN.UTHSCSA.EDU
FAX No. for Branson Sonicators Duncan Clark
Storage for PCR Dan McGoldrick
- Storage for PCR Dan Oerther
The roles of gelatin, tween20 and glycerol in PCR Alex Chang
- The roles of gelatin, tween20 and glycerol in PCR szcooley at chip.ucdavis.edu
Biodegradable substance Karine Gabrielian
annealing temps. for PCR HARDIES at THORIN.UTHSCSA.EDU
Peptide mapping Ravi Ramachandran
- Peptide mapping Dennis Templeton
Disappearing Plasmids--Help! Carlisle Landel
- Disappearing Plasmids--Help!
- Disappearing Plasmids--Help! Dennis Templeton
  - Disappearing Plasmids--Help! anon
    - Disappearing Plasmids--Help! Dennis Templeton
ds Sequencing Qiagen-prep Plasmids-Help! Carlisle Landel
- ds Sequencing Qiagen-prep Plasmids-Help! fgrun
Test lq at dna.warwick.ac.uk
ds sequencing Quiagen plasmid preps plxigpg at vax.ccc.nottingham.ac.uk
Deiodinase Type II cDNA Aaron Pawlyk
Pfu Poly, Cloning ez001427 at dale.ucdavis.edu
- Pfu Poly, Cloning Hong Dang
- Pfu Poly, Cloning Duncan Clark
RNA from skin + uterus, protein contamination woes acer
Perkin Elmer Gene AMP? Shahram Mori
TnT system in vitro translation Stefan Gruenert (WCI)
Quick Coomasie Destain Allison Haggarty
PCR to differentiate meats Viraj Master
Current Contents online Duncan Clark
- Current Contents online Uwe Harmening
  - Current Contents online Dave Smith
Expression in Pichia? f_benson at icrf.icnet.uk
TGF-beta expression vector wanted G Gallagher
- ATTN KGEISS / AAMRL TGF-beta expression vector wanted G Gallagher
How well does Taq survive in a frost free freezer? Anthony C.
- How well does Taq survive in a frost free freezer? Brett Beitzel
  - How well does Taq survive in a frost free freezer? Cornelius Krasel
    - How well does Taq survive in a frost free freezer? Duncan Clark
- How well does Taq survive in a frost free freezer? Hey leave me alone!
  - mini-prep DNA isolation procedures Paul N Hengen
- How well does Taq survive in a frost free freezer? Zophonias O. Jonsson
  - How well does Taq survive in a frost free freezer? Anthony C.
Cloning PCR products: polish ends. Brian Foley
Does pUC18 have a T7 promoter? Tracy Aquilla
Does pUC18 have a T7 promoter? Mark Farinha
RNA oligos? Michael Powell
Isotope Incorporation? HELP ez001427 at dale.ucdavis.edu
in-vitro vs. in-vivo translation Weixing Luo
Coomassie Blue G-250 staining Unknown
- Coomassie Blue G-250 staining Elmer M. Price
- Coomassie Blue G-250 staining neeraj jain
Antibody clones H. David Fischer
How well does Taq survive in a frost free freezer? fgrun
5'-AmpliFINDER RACE Kit CLONTECH at BIOTECHNET.COM
- 5'-AmpliFINDER RACE Kit Paul N Hengen
Benchtop shaker-incubators Danuta Bois
PCR questions? LEES at SASK.USASK.CA
- PCR questions? Jim Owens
- PCR questions? Duncan Clark
- PCR questions? Andy Law Big Nose
metallothionine promoter Bulletin board login
- metallothionine promoter Unknown
pea genomic library needed CHRISTOPHER M. SMITH
protocols on CnBr clivage of peptids Clement-Major Sebastien
- protocols on CnBr clivage of peptids Alexander P Rostovtsev
Coomassie Blue G-250 staining Dietmar Tietz
- Coomassie Blue G-250 staining Paul N Hengen
Overexpression of large proteins Robert Hindges
Looking for Chicken Genome Library Nancy Shaper
genomic DNA purification questions Frederick Garbrecht
- genomic DNA purification questions nishir at ohsu.edu
- genomic DNA purification questions Nicola Fidler
- genomic DNA purification questions Paul N Hengen
  - genomic DNA purification questions Wiggy
- genomic DNA purification questions Artur Burzynski
XL-1 Red---Beware! Scott Vande Pol
- XL-1 Red---Beware! Brian Foley
Error in Pharmacia pGEX Instructions Alan Gerstein
Oligo Cloning Seth Findley
Measuring Pi to determine ATPase:help Koller at koller
Coomassie Blue G-250 staining Marion Freistadt
- Coomassie Blue G-250 staining Elmer M. Price
  - Coomassie Blue G-250 staining R.G. Walters
- Coomassie Blue G-250 staining Robert Slany
- Coomassie Blue G-250 staining Tracy Aquilla
- Coomassie Blue G-250 staining Tracy Aquilla
- Coomassie Blue G-250 staining Paul N Hengen
DNA isolation from low melting agarose blocks Miss C.I. Kwok
Ultralow recommendations needed Parke K. Flick
ssDNA circularization and T4 RNA ligase? Inna Vainshtein
Coomassie Blue G-250 staining ez001427 at dale.ucdavis.edu
trangene newsgroup/discusion group mluo at cc.memphis.edu
HA antibody 12CA5 availability? Dennis Templeton
avidin Graham Collins
- avidin Shiao Y. Wang
  - Use of Streptavidin coated beads Paul N Hengen
  - affinity purification of receptor (was Re: avidin) Bryan Crawford
    - affinity purification of receptor (was Re: avidin) Cornelius Krasel
  - avidin T. S. Pillay
Different types of PCR Stringer, Peta
- Different types of PCR Eric C. Anderson
HA antibody 12CA5 availability? Jim Woodgett
DNA isolation from low melting agarose blocks Antje Haase
YAC rat library available? Allison Haggarty
Cracking SDS gels Mike Morris
- Cracking SDS gels Ashok Aiyar
  - Cracking SDS gels Chris Larsen
- Cracking SDS gels Annette C. Hollmann
qiagen plasmid preps Peter S. Silverstein
- qiagen plasmid preps Artur Burzynski
- qiagen plasmid preps Andy Law (Big Nose)
  - qiagen plasmid preps Robert Woodward
ligation in LMA Clemens Suter-Crazzolara
- ligation in LMA G Gallagher
fragment isolation w/o glass milk? Compilation Daniel Schlieper
qiagen plasmid preps Stephen R. Lasky
- qiagen plasmid preps B Stickney
two-hybrid cDNA libraries-fly and vertebrates? padgett at mbcl.rutgers.edu
cracking SDS PAGE brett at BORCIM.WUSTL.EDU
PCR questions (the overhang) HARDIES at THORIN.UTHSCSA.EDU
qiagen plasmid preps Matthias Zeiner
gravimetric detection of oligonucs borges at almaden.ibm.com
- gravimetric detection of oligonucs Shiao Y. Wang
frost-free freezer blues HARDIES at THORIN.UTHSCSA.EDU
SV-40 Promoter Bashing Joe M. SandyFaulkner, Jr.
- SV-40 Promoter Bashing David S. Huen
(none) Allan Gibson
Guidelines for Tissue Culture Facility Brian T. Greuel
Overexpression of large proteins Clemens Suter-Crazzolara
Looking for a Public Domain MSDS Sheet Server Greg Carter
- Looking for a Public Domain MSDS Sheet Server Ashok Aiyar
Disappearing Plasmids--Help! contente at usuhs.usuhs.mil
RNA elution/secon. structure otello at sc2a.unige.ch
- RNA elution/secon. structure user at mole.bio.cam.ac.uk
unsubscribe Allan Gibson
genomic DNA purification questions contente at usuhs.usuhs.mil
Qiagen/Nucleobond Cartridge Reuse Amos H Heckendorf
- Qiagen/Nucleobond Cartridge Reuse Jim Woodgett
- Qiagen/Nucleobond Cartridge Reuse Paul N Hengen
How well does Taq survive in a frost free freezer? Klaus.Matthaei at ANU.EDU.AU
partial library chang at GSBS18.GS.UTH.TMC.EDU
- partial library John Nash
  - partial library Paul N Hengen
jk rob
DS DNA Sequencing Help VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
Help needed for unstable plasmid construction Bernard Murray
- Help needed for unstable plasmid construction szcooley at chip.ucdavis.edu
  - Help needed for unstable plasmid construction Ian A. York
- Help needed for unstable plasmid construction Stuart Kuhstoss
- Help needed for unstable plasmid construction Klaus Salger
Sigma Electrophoresis Unit and Gel "Smiles" MICHAEL F. FREY
Thrombin cleavage problem Graham Atherton
- Thrombin cleavage problem Jim Woodgett
- Thrombin cleavage problem Bipin K Dalmia
RNAse stability Robert Slany
RNA stuck in the gel wells Grad Student Lab 2
FISH practicioners in UK. daj (David Johnston)
antibodies against IL6 signaltransducing gp130 anywhere?? Horst Ibelgaufts
Taq with Triton X-100, DMSO wmelchior at fdant.nctr.fda.gov
PCR primers for mouse IFNAR? Mary Coussons-Read
unstable plasmid construction HARDIES at THORIN.UTHSCSA.EDU
sequencing kits? John Brunstein
Re Thermal Cyclers Kevin O'Donnell
Re Thermal Cyclers Roger Wiegand
metallothionine Lauri Lintott
SYBR Green I Jeanne Loring
- SYBR Green I Scott Selby Med Heme
genomic DNA purification questions: protocol contente at usuhs.usuhs.mil
Pichia vectors Virginia Dress
- Pichia vectors Jeffrey Shane Temple
Directional In-Gel Cloning John Watson
DEPC in RACE? Harry Menegay
- DEPC in RACE? John Watson
List of GRAS (Generally Recognized as Safe) microorganisms Gabriel Dorado
phone # inquiry David Char
unsubscribe Parin
Server for MSDS Shaun D. Black
Competitive PCR - Questions Carl Waltenbaugh
- Competitive PCR - Questions douglas l feinstein
  - Competitive PCR - Questions szcooley at chip.ucdavis.edu
- Competitive PCR - Questions douglas l feinstein
Gel-Reading Programs Todd Giles
- Gel-Reading Programs Anthony Tomlinson
Matchmaker 2-Hybrid Kit Christie Doris Zerylnick
re-amplication of PCR product without internal primers BHWFDUNG at CPHKVX.CPHK.HK
- re-amplication of PCR product without internal primers Hong Dang
- re-amplication of PCR product without internal primers douglas l feinstein
- re-amplication of PCR product without internal primers Andy Law Big Nose
DIG nonradioactive kit to screen cDNA library Xin-Hua Zhou
Sequencing near poly A - help Shiao Y. Wang
2-D N/N Agarose Gels Anthony Smith
RNAse stability Clemens Suter-Crazzolara
pGEX GST Fusion Question... Simon Twigger
CTG as a start codon mazella
PMMA cover slips KDAVIES
Lux expression conditions?? MOUNCEY
CIP Michael G. Tencza
- CIP Peter Gegenheimer
  - CIP Bernard Murray
sequencing near poly(A) tail HARDIES at THORIN.UTHSCSA.EDU
Sequencing near poly A - help Douglas Rhoads
RNAse stability HARDIES at THORIN.UTHSCSA.EDU
- RNAse stability Peter Gegenheimer
Potent denaturants Wolfram Brune
CTG as a start codon David Glover
seq polyA mcgraw at BSCR.UGA.EDU
RNA Ali Riazi
- RNA Michael van Waes
RNA extraction Ali Riazi
(none) lylee at BULLWINKLE.UCDAVIS.EDU
potent denaturants HARDIES at THORIN.UTHSCSA.EDU
restriction site mapping program needed gotow lisa fujiko
- restriction site mapping program needed ez001427 at dale.ucdavis.edu
- restriction site mapping program needed Manuel G. CLAROS
- restriction site mapping program needed Manuel G. CLAROS
Amino acid grouping Shaojiu Wang
- Amino acid grouping Brian Smith-White
QUIAGEN SPAGNOL at PLAUTO.CSATA.IT
Dyna-magnetic DNA probe for Southern Kah-Tong, Seow
- Dyna-magnetic DNA probe for Southern Matthew Wakefield
Fuzzy sequence gel bands Bengt Oxelman
Fuzzy sequencing gel bands Bengt Oxelman
- Fuzzy sequencing gel bands Robert Horton
  - Looking for Haolin Ni Zainul Zain
- Fuzzy sequencing gel bands Frosty Loechel
  - Fuzzy sequencing gel bands Dan Diaz
PCR of SuperScript 1st Strand? gustilo at pobox.upenn.edu
- PCR of SuperScript 1st Strand? Richard Near
AFP clone available? Allison Haggarty
bitter sugar SPAGNOL at PLAUTO.CSATA.IT
- bitter sugar Virginia Dress
- bitter sugar Brian Smith-White
Effects of SDS on Thermolysin? Stallion
Ultralow recommendations needed Curt Ashendel
regulations on transgenic plants Gary Kuleck
Porcine liver cDNA library Kurihara Tatsuo
Electrofusion of plant proto's via BIORAD Gene Pulser?? Jeff
Assay for biotin-PCR products Shiao Y. Wang
- Assay for biotin-PCR products Roger Wiegand
Making proteinase K stock Shiao Y. Wang
Porcine cDNA Library Kurihara Tatsuo
Chromosome probes for ISH Marie-Terese Little
RNase stability rhubner at molbiol.ox.ac.uk
HepG2 cells and corticosteroids Matthias Zeiner
erbB3 and erbB4 spiff at psycho.toppoint.de
- erbB3 and erbB4 Tracy Aquilla
ds Sequencing with Qiagen prep Dr. C.J. Chan
PCR primers for mouse IFNAR? (fwd) Mary Coussons-Read
Transformation problems Emma Macfarlane
- Transformation problems ez001427 at dale.ucdavis.edu
- Transformation problems Tracy Aquilla
- Transformation problems Brian Foley
Oligo that won't label Ms I G Zvetkova
- Oligo that won't label Brian Smith-White
E.coli transformation protocol mccallo at rand
- E.coli transformation protocol Rafa Maldonado
- E.coli transformation protocol S. LaBonne
- E.coli transformation protocol Peter Alefounder Bioc
plasmid recovery from "dead" plates Kimberly Ann Winkeler
NEED: Clontech 5' RACE kit Protocol Eric C. Anderson
fuzzy sequencing gel bands (glycerol?) HARDIES at THORIN.UTHSCSA.EDU
NEED: Clontech 5' RACE kit Protocol Eric C. Anderson
Does anyone have pGEX-KN/pGEX-KT, please? Viraj Master
Spin Coluumns Peter E. Prevelige
Differential scanning calorimetry Alan Simmons
- Differential scanning calorimetry Eric C. Anderson
Plasmid size limitation on E. Coli Transformation? BrianC4208
- Plasmid size limitation on E. Coli Transformation? Steve Minchin
Magnetic beads staff. Reusable??? Dave Knorr
Plasmid size limitation on E. Coli Transformation? Mark Farinha
- Plasmid size limitation on E. Coli Transformation? elliott s. goldstein
- Plasmid size limitation on E. Coli Transformation? Paul N Hengen
gel sealing tape nishir at ohsu.edu
- gel sealing tape Karl A. Suiter
- gel sealing tape Brian Smith-White
Magnetic beads staff. Reusable??? A428ENDE at VM1.SARA.NL
size limit to transformation HARDIES at THORIN.UTHSCSA.EDU
fuzzy sequencing gel bands (glycerol?) ez001427 at dale.ucdavis.edu
In situ PCR of mitochondrial DNA Jim Cummins
Chromosome probes for ISH Marie-Terese Little
TAQ contaminated with DNA? David Adelson
(none) Wafa.El-Adhami at ANU.EDU.AU
TAQ contaminated with DNA? magoldstein at ucdavis.edu
- TAQ contaminated with DNA? John Nash
  - TAQ contaminated with DNA? Paul N Hengen
    - TAQ contaminated with DNA? Paul N Hengen
- TAQ contaminated with DNA? Duncan Clark
  - TAQ contaminated with DNA? Wolfgang Kusser
- TAQ contaminated with DNA? Paul N Hengen
Gel sealing tapes Dr. C.J. Chan
Taq contamination? berschic at convex.rz.uni-duesseldorf.de
taq contamination? berschic at convex.rz.uni-duesseldorf.de
D.N.A PURIFICATION......HELP PLEASE MED6MHS at VAX.BIO.LEEDS.AC.UK
- D.N.A PURIFICATION......HELP PLEASE Mike Morris
- D.N.A PURIFICATION......HELP PLEASE Yasha Hartberg
optimizing PCR reactions Frederick Garbrecht
- optimizing PCR reactions szcooley at chip.ucdavis.edu
  - Touchdown PCR reactions Peter Kaub
    - optimizing PCR reactions Ron Kagan
- optimizing PCR reactions Mr V. Schoenfeld DOM
Alternatives to 32P for Phosphorylation States? Stefan M. Gorsch
- Alternatives to 32P for Phosphorylation States? Eric C. Anderson
- Alternatives to 32P for Phosphorylation States? T. S. Pillay
plasmid recovery from "dead" plates Kimberly Ann Winkeler
- plasmid recovery from "dead" plates Paul N Hengen
- plasmid recovery from "dead" plates Rafa Maldonado
- plasmid recovery from "dead" plates Eric C. Anderson
  - plasmid recovery from "dead" plates Paul N Hengen
- plasmid recovery from "dead" plates Eric C. Anderson
(none) jd89
need help to purify antimicrob agnts bartholo at aardvark.ucs.uoknor.edu
Fixing sequencing gels. John Maris
- Fixing sequencing gels. Suzy Schneider
- Fixing sequencing gels. Tracy Aquilla
No Subject Wolfram Brune
vancomycin and Agrobacterium Bjorn Orvar
- vancomycin and Agrobacterium szcooley at chip.ucdavis.edu
PCR inhibitors in insect tissue? edwardsg at skyfox.usask.ca
- PCR inhibitors in insect tissue? Tracy Aquilla
gel sealing tape Bruce Elder
- gel sealing tape Yasha Hartberg
Excision of Bluescript from Lambda ZAP Michael Lydan
subscribe Charles L. Rutherford
subscribe methods Charles L. Rutherford
unsubscribe Charles L. Rutherford
Alternatives to 32P for Phosphorylation States? Curt Ashendel
PCR optimization HARDIES at THORIN.UTHSCSA.EDU
sealing sequencing gels HARDIES at THORIN.UTHSCSA.EDU
hplc column for peptide analysis Walt Lilly
- hplc column for peptide analysis Paul Donald Pyla
  - hplc column for peptide analysis Azlinda Bte Anwar
FAQ list for bionet.molbio.methds-reagnts Paul N Hengen
Best molbio software package? Tracy Aquilla
- Best molbio software package? szcooley at chip.ucdavis.edu
  - Best molbio software package? Sean David Moore
- Best molbio software package? T. S. Pillay
  - Best molbio software package? Kevin Morano
    - Best molbio software package? T. S. Pillay
- Best molbio software package? Yasha Hartberg
plasmid library Jim Gale
Ortho Diagnostics address? uchmtdm at EARTH.OSCS.MONTANA.EDU
E. coli PFGE Rafa Maldonado
- E. coli PFGE Tracy Aquilla
Excision of Bluescript from Lambda ZAP John Watson
gel sealing tape John Watson
HLA-DQ typing Wachara Kasinreak
size limit to transformation Slawek Bartoszewski
Plasmids in the dark.. GONCZOL at WISTA.WISTAR.UPENN.EDU
Calling GERMANY: Chemie-Sicherheitsdaten Horst Ibelgaufts
Do any journals take papers on disc? Graham Atherton
- Do any journals take papers on disc? Ian A. York
  - Do any journals take papers on disc? Kevin Morano
plasmid size limitation for transformation GWDGV1::KCHOWDH
Large scale microsatellite screening Dan Bradley
L lactis lactis CNRZ270 Christopher Coward Path
E. coli PFGE Ted Davis
ATTN: Jaz: Clontech 5' RACE Protocol Eric C. Anderson
sealing gels-shocking news HARDIES at THORIN.UTHSCSA.EDU
selection of dimeric proteins using filamentous phage Stephane Vuilleumier
Northern analysis with Renaissance Kit GUT at QUCDN.QueensU.CA
T7 RNA polymerase promoter question. Paul N Hengen
large plasmids brett at BORCIM.WUSTL.EDU
- large plasmids Rafa Maldonado
- large plasmids Tracy Aquilla
mRNA storage J.P. Hays
- mRNA storage Andre Hamel
- mRNA storage Peter Gegenheimer
- mRNA storage Tracy Aquilla
mRNA storage Lisa J Brunet
mRNA storage Hanson Lab
fixed my plasmid problem, thanks Hanson Lab
T7 RNA polymerase promoter question. jr at dna.bio.warwick.ac.uk
- T7 RNA polymerase promoter question. Duke Groebe
- T7 RNA polymerase promoter question. Peter Gegenheimer
gel sealing tape Norman J. Pieniazek x4073
- gel sealing tape Ashok Aiyar
TAQ contaminated with DNA? (fwd) Robert Preston
- TAQ contaminated with DNA? Duncan Clark
mRNA storage NEAL MUSTO
Manual for DNAsis-Mac V.2.0 KY HWA
biotechnology jurgenson at cobra.uni.edu
transfection of T-cells user at ichr.uwa.edu.au
- transfection of T-cells Mike Klopfer
- transfection of T-cells Jonathan M Kaplan
re-amplication of PCR product without internal primers Klaus.Matthaei at ANU.EDU.AU
Mac freeware for binding? Hong Dang
<None> rosas at mcclb0.med.nyu.edu
Subcloning dirty PCR products rosas at mcclb0.med.nyu.edu
- Subcloning dirty PCR products Dr. YL Du
- Subcloning dirty PCR products Rafa Maldonado
  - Subcloning dirty PCR products Ian A. York
- Subcloning dirty PCR products Tomas Bratt
- Subcloning dirty PCR products schupp at nauvax.ucc.nau.edu
Is downward transfer REALLY good for Northern? Jason Lei
- Is downward transfer REALLY good for Northern? nishir at ohsu.edu
Manual for DNAsis-Mac V.2.0 magoldstein at ucdavis.edu
Amplified Refractory Mutation System PCR Jim Cummins
site directed mutagenesis Bulletin board login
- site directed mutagenesis Rafa Maldonado
Myotube nuclei John Chesters
Insulin receptors Pinter Shlomit
- Insulin receptors T. S. Pillay
Subcloning dirty PCR products Emma Macfarlane
Library screenings by PCR raul arredondopeter
- Library screenings by PCR Eric C. Anderson
casein overlay procedure "Christine Pull ", BIO
Help! "Hot" PCR probe for Northern Blots? Jinling Xie
Two-dimensional DNA electrophoresis Richard Levenson
- Two-dimensional DNA electrophoresis Antje Haase
  - Two-dimensional DNA electrophoresis John Brunstein
slow growing cultures MUNGO at UVVM.UVIC.CA
Help! "Hot" PCR probe for Northern Blots? Mr V. Schoenfeld DOM
FEARFUL SIMMETRY SPAGNOL at PLAUTO.CSATA.IT
RNAse inhibitor Janet E. Rubin
Library screening by PCR Emma Macfarlane
RNAse inhibitor Lisa J Brunet
- RNAse inhibitor B Stickney
Differential Display PCR/ Pulse field gel electro phoresis questions Jack M. Bernstein, M.D.
help: phage DNA extraction pzhang at CC.UManitoba.CA
protein calorimetry Jordan Konisky
gel sealing tape nishir at ohsu.edu
help: phage DNA extraction John Nash
Subcloning dirty PCR products Steve
Teosinte library raul arredondopeter
Protein complex dissociation nievesr at TCPLINK.NREL.GOV
RNA REMOVAL: Help! ez001427 at dale.ucdavis.edu
- RNA REMOVAL: Help! Hanson Lab
- RNA REMOVAL: Help! Michael van Waes
- RNA REMOVAL: Help! ez001427 at dale.ucdavis.edu
  - RNA REMOVAL: Help! Jim Owens
- RNA REMOVAL: Help! Dongxian Yue
affinity chromatography nievesr at TCPLINK.NREL.GOV
site directed mutagenesis ez001427 at dale.ucdavis.edu
- site directed mutagenesis Ashok Aiyar
pMEXneo sequence Toru Imamura
restriction enzyme list David N. Smith
- restriction enzyme list
restriction enzyme list magoldstein at ucdavis.edu
restriction enzyme list Chaisiri Wongkham
"Cold" SSCP Chris Weghorst
summary: re-amplification of PCR product using the original BHWFDUNG at CPHKVX.CPHK.HK
- summary: re-amplification of PCR product using the original Muriel Liberto
vancomycin and Agrobacterium Wolfram Brune
- vancomycin and Agrobacterium Stuart Kuhstoss
  - vancomycin and Agrobacterium szcooley at chip.ucdavis.edu
expression vector pT7-7 or derivative Ursula Schell
E.coli transformation protocol wanted berry at biochem.unp.ac.za
Antisense oligos Dr. C.P. Healy
Oligo synthesis woes Dr. C. Dolphin
HPLC coloumn for peptides berry at biochem.unp.ac.za
RNA REMOVAL: Help! Douglas Rhoads
e.coli transformation question Andreas Weber
- e.coli transformation question Duke Groebe
- e.coli transformation question Rafa Maldonado
Antisense oligo design Dr. C.P. Healy
High MW protein standards Dave Smith
- High MW protein standards T. S. Pillay
fos and jun Gotz von Bulow
- fos and jun Tracy Aquilla
Problem with inversed binding on a dot blot doug white
- Problem with inversed binding on a dot blot Andreas Weber
In Search of Beta Sites : (help??) MBPI at BIOTECHNET.COM
Micro Journal Club Tom Schneider
Sma I degrading ends (an alternative) HARDIES at THORIN.UTHSCSA.EDU
To Georges from France, Hong Dang
subcloning dirty pcr products Emma Macfarlane
- subcloning dirty pcr products Torben Osterlund
Sequencing Gel Spacer Plastic Anthony Otsuka
Cloning in Afl III site in pUC vectors Duncan Clark
- Cloning in Afl III site in pUC vectors Duncan Clark
Afl III site in pUC vectors Duncan Clark
- Afl III site in pUC vectors Duncan Clark
Growing Up TA Cloning Products Andrew McArthur
- Growing Up TA Cloning Products Lisa J Brunet
  - Growing Up TA Cloning Products Duncan Clark
    - Growing Up TA Cloning Products Carlisle Landel
- Growing Up TA Cloning Products douglas l feinstein
- Growing Up TA Cloning Products Chong-Yee Khoo
Differential Display PCR Michael Joiner
mRNA storage Seth Corey
- mRNA storage pgegen at RNAWORLD.BIO.UKANS.EDU
  - mRNA storage B Stickney
- mRNA storage John Watson
Hints for low-stringency cloning Tae Bum Shin
- Hints for low-stringency cloning Gill Diamond
- Hints for low-stringency cloning John Watson
- Hints for low-stringency cloning Doc Martin
cDNA library kits, which is best? Richard Dana
- cDNA library kits, which is best? douglas l feinstein
ß-galactosidase and NP-40 Karl Fischer
- ß-galactosidase and NP-40 schupp at nauvax.ucc.nau.edu
Why PCR reamp = smears. HARDIES at THORIN.UTHSCSA.EDU
in vitro mutagenesis David Adelson
- in vitro mutagenesis Yasha Hartberg
Promoter and DNA consensus-finding software Ron Kagan
HPLC column for peptides MAHER DERBEL
NORTHERN BLOTS Philip OBrien
dihydordiol purchase ?? Lee
Protein electroelution from SDS-page T. S. Pillay
- Protein electroelution from SDS-page Roland J Saldanha
Growing up TA cloning products Graham Atherton
pseudogene Dr. B. Sylla
Detection of H3 expression (fwd) vetfisio at IPRUNIV.CCE.UNIPR.IT
PCR from Fibroblasts? Scott Wildenberg DGM-IHG
tRNA nucleotidyl transferase Jitao
PCR from Fibroblasts? Mike Morris
Detecting mutations. Dr. G.R. Taylor
Densitometry software? Mr TG Downing
- Densitometry software? schupp at nauvax.ucc.nau.edu
- Densitometry software? Robert Horton
glycosylated protein isolation jgill at kean.ucs.mun.ca
Why PCR reamp = smears. (fwd) Robert Preston
- Why PCR reamp = smears. (fwd) szcooley at chip.ucdavis.edu
tRNA nucleotidyl transferase Jitao
Poly A RNA Grant McKegney
HELP with sequencing acc NAR 16(22):10915 David Johnson
HELP with sequencing acc NAR 16(22):10915 David Johnson
anthocyanins and protein isolation Nokwanda Makunga
- anthocyanins and protein isolation Peter Gegenheimer
RNA isolation from small # of cells? bormann at urz.unibas.ch
Cloning Repetitive DNA WATSONC at QUCDN.QueensU.CA
PCR from intact bacterial cells mohanish at pharmdec.wustl.edu
- PCR from intact bacterial cells Marc A. Goldstein
  - PCR from intact bacterial cells Julian Dow
- PCR from intact bacterial cells Robert Hindges
Why PCR reamp = smears. HARDIES at THORIN.UTHSCSA.EDU
Why PCR reamp = smears. (fwd) Klaus.Matthaei at ANU.EDU.AU
hIL-4Ralpha primers: suggestions please davis at wehi.edu.au
direct sequencing from chromosome? adler at staff.monash.edu.au
anthocyanins and protein isolation William.Cress
S:avrI and sciI; recognition&cut? Professor Rudolf Prof. Dr. Eichenlaub
- S:avrI and sciI; recognition&cut? Mark Farinha
Nonisotopic AA transport assay? Hr Dr. S. Shapiro
RNA from E.coli Bulletin board login
- RNA from E.coli Cornelius Krasel
Medical Genetics management s/ware Mike Morris
temp.sensitive ori / pBR322 deriv. FJ van der Wal
General note on distribution of vectors strains etc. Duncan Clark
Microwave Miniprep - protocol? Robert Rumpf
Buying Hyb. Oven/Roller ktgeiss at miavx1.acs.muohio.edu
Biotin in hybridisation. Dr. A. Rosenthal
membrane preparation from COS cell Georges Gaudriault
DNA from lypholized tissue? szcooley at chip.ucdavis.edu
PGEM accession numbers Tod Dwayne Strugnell
pGEM accession numbers Tod Dwayne Strugnell
- pGEM accession numbers ez001427 at dale.ucdavis.edu
Plasmid size limitation Cynthia Shippam
Gel sealing tapes Cynthia Shippam
Gel sealing tapes Cynthia Shippam
Rapp Polymere resin smithr at MRC.COM
Buying Hyb. Oven/Roller lehn at facstaff.wisc.edu
Where can I get DNA Strider 1.2? Tracy Aquilla
- Where can I get DNA Strider 1.2? Pierre Martineau
Buying Hyb. Oven/Roller Mark Farinha
rescue dead stock elliott s. goldstein
T7 Expression System Klara Briknarova
- T7 Expression System Bernard Heymann
- T7 Expression System ez001427 at dale.ucdavis.edu
Primers with dI vs mixed bases? Allen Gathman
HBSAG Salvatore Sechi
Buying Hyb. Oven/Roller James S. Sutcliffe
PI values Richa Behari
wheat library for 2-hybrid system Richard Dana
Looking for mol bio software.... Frank White
PNK substrates Dongxian Yue
Any experience with Soccorex pipettors Simon Ginsberg
- Socorex pipettes Mike Morris
Ultralow freezer - summary Shiao Y. Wang
(none) 126DANIE at WITSVMA.WITS.AC.ZA
(none) Gotz von Bulow
Any experience with Soccorex pipettors daj (David Johnston)
where is Cambridge Res. Biochem.? smithr at MRC.COM
Sequencing using infrared tags Ann Kleinschmidt
Problem getting JM110 competent Tom
where is Cambridge Res. Biochem.? Dr R. Dalgleish
How to treat cells in interphase stage for FISH? Wasun Chantratita
long range PCR m-brazil at nimr.mrc.ac.uk
lambda/HindIII B Stickney
- lambda/HindIII Andrew Cockburn
protein elution Tzahi Arazi
Oddity on sequencing gels Bruce Elder
where to get Rapp Polymere TentaGel? smithr at MRC.COM
plasmid drawing shareware? gr8bn at cc.usu.edu
SYBR Green I, more experiences Jun Kim
info about cosmids Jeffrey D. Bray
Inexpensive blotting paper? Dennis Templeton
SYBR Green I, again Jun Kim
PCR with 3' mismatch HARDIES at THORIN.UTHSCSA.EDU
RC20 anti-phosphotyrosine Ab: non-specific detection William R. Morgan
rescue dead stock Rachelle Leger
protein elution Klaus.Matthaei at ANU.EDU.AU
PCR from intact bacterial cells Rachelle Leger
Taq DNA Pol and frameshifts Michael J Marino
Oddity on sequencing gels ez001427 at dale.ucdavis.edu
T7 Expression System Richard T. Timmer
ABI Sequencer Facility management? We've recently set up an ABI 373 sequencer in a shared facility. Any net suggestions as to software to simplify the MANAGEMENT of the facility (i.e., Excel spreadsheet templates for sample entry, automated mailing of users results, etc,,,)? Seems like this issue may have been dealt with before. I've already heard of Toolkit... Thanks in advance for any info, Richard R. Hardy
RE- Soccorex pipettors Dieter Palmer

Last message date: Wed Aug 31 22:44:08 EST 1994
Archived on: Sun Mar 6 20:10:47 EST 2005

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