plasmid vectors for yeast two-hybrid system?

bcraft at bcraft at
Thu Dec 1 23:39:08 EST 1994

Can anyone tell me what are the latest, or preferably, the best plasmid 
vectors, etc. to use for the yeast two-hybrid system.  I know that some 
companies are making kits (Clontech, I think), but what else is out there?

I currently have pGAD2F and pMA424 which are large unwieldy vectors.  I know 
there are pACT and pAS1 which are smaller and newer/better(?), are these any 

Brian Craft
bcraft at

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