fuzzy pcr

"PBS::NELSONJ" at PBS.DFO.CA "PBS::NELSONJ" at PBS.DFO.CA
Thu Dec 1 18:42:45 EST 1994



Hi,
	I am doing microsatellite amplification from genomic dna and I find
for one of the microsatellites I'm amplifying the bands (around 100-200 bp)
always seem to be fuzzy on a 3% nusieve/1% BRL gel.  I don't think it is the gel
as the marker looks ok and other microsatelites amplified of approx this size
amplify up as more distinct bands.
	Any ideas out there on this?  
John         nelsonj at pbs.dfo.ca









More information about the Methods mailing list