DNase with activity at alkaline pH?

Roger Graham flo at unixg.ubc.ca
Fri Dec 2 17:49:49 EST 1994


Dear Netters :

I would like to enzymatically cleave plasmid DNA at pH 9-10; specifically I
would like to show protection/cleavage of my DNA from "DNaseI"-type
activity in the presence/absence of my complex. Unfortunately, neutral pH
dissociates my complex. Any suggestions for a candidate nuclease ?

Thanx,

-- 
Roger Graham
Dept. of Biochemistry
University of British Columbia
Vancouver, B.C., Canada
email : flo at unixg.ubc.ca



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