use of spermidine to remove DNA from prot. solutions

Alberto I Roca roca at macc.wisc.edu
Fri Dec 2 12:59:56 EST 1994


In article <aquilla.1136606835F at sadye.emba.uvm.edu> Tracy Aquilla,
aquilla at salus.med.uvm.edu writes:
>a protocol or can suggest a reference, we'd be very grateful. Thanks.

while purifying a RecA mutant, these authors used 5mM spermine acetate
to precipitate nucleic acids. see

Dutreix, M., Burnett, B., Bailone, A., Radding, C. M., and Devoret, R.,
A partially deficient mutant, recA1730, that fails to form normal
nucleoprotein filaments, Mol Gen Genet, 232, 489, 1992.

alberto :|
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Alberto I. Roca                 Internet: roca at macc.wisc.edu
Biochemistry                    Bitnet:   roca at wiscmacc
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