DNase FOOTPRINTING Off a Gel-Retarded Complex
Sandy Curtiss
swcurt at ccmail.monsanto.com
Fri Dec 2 09:57:47 EST 1994
In article <3bde8u$l6p at bigfoot.wustl.edu> Thiruvamoor P. Ramkumar,
ramu at artsci.wustl.edu writes:
>Hi,
> i'm looking for help on doing DNase Footprinting from a
>DNA-Protein complex isolated from a gel-retarded complex. Primarily, my
>concern is to elute the DNA-Protein complex out of the gel without
>disrupting the complex. Possible buffer compositions, elution
conditions,
>quantitation parameters etc. will all help.
>
>
> _
>ramu
A protocol for doing DNase footprinting directly in a mobility shift gel
is given in Kuwabara, MD and Sigman, DS (1987) Biochemistry 26:
7234-7238. I have not used this protocol, but it was used successfully
by Wolfe et al. (1993) JBC 268: 12418-12426. Hope this helps.
Sandy Curtiss
Monsanto Co.
swcurt at ccmail.monsanto.com
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