Techne Gene E thermocyclers

dpetersn at unixg.ubc.ca dpetersn at unixg.ubc.ca
Fri Dec 2 14:59:24 EST 1994


In article <3b2npq$etq at mserv1.dl.ac.uk> tculling at hgmp.mrc.ac.uk (Dr. T.E. Cullingford) writes:
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>From: tculling at hgmp.mrc.ac.uk (Dr. T.E. Cullingford)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: Techne Gene E thermocyclers
>Date: 24 Nov 1994 18:59:06 -0000
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>Original-To: methods at dl.ac.uk


>We have recently purchsed two of the above machines, without heated
>lids, from Techne. I have found that, using an external thermistor
<<<stuff deleted>>>

Some time agor I wrote regarding our similar problems with lag times on 
our Techne thermocycler.  (Not to mention the breakdown of three blocks within 
a 1 year period.)  This problem cannot be changed in anyway I am aware of, 
except by changing instruments.  PCR failures due to lag times cannot be 
differentiated from inconsistent well-to-well temperature variability, or lack 
of template, etc.





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