Tricine buffer for PCR

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Sat Dec 3 13:16:56 EST 1994


szcooley at chip.ucdavis.edu (Michael Cooley) writes:

>I hear tell of a buffer for PCR based on Tricine. It's supposed to hold a 
>higher pH at the elevated temperatures of the PCR. Has anyone tried this 
>and does anyone know the recipe for making it?

Check out Nucleic Acids Research, 20, 623 for a comparison of buffers 
containing Tris vs Tricine.

-- 
Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk                               



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