advice on phosphorimager purchase

Tracy Aquilla aquilla at salus.med.uvm.edu
Sat Dec 3 14:38:46 EST 1994


In Article <3bitqj$cs3 at newsbf01.news.aol.com>, tbannor at aol.com (T Bannor) wrote:
>Here are a couple of helpful hints.
>
>1. Always wrap your wet samples with Saran Wrap (the real stuff from Dow).
>This passes less moisture than any other wrap and should keep your plate
>from being damaged. If you are worried about quenching of the signals you
>can use very thin aluminized mylar which may be available from Fuji at
>this time, if not, they can tell you where to get it.

I have noticed that when placing blots wrapped with Saran in the loading
dock for exposure, sometimes the plates get contaminated. This can be a real
problem for multiple users. I now use seal-a-meal bags instead, since they
don't leak.

>
>2. In these instruments the only thing that 16 bits gets you is the
>ability to pull faint signals out of the noise. The Fuji instruments have
>a superior signal to noise ratio, which is why they can get away with
>using 8 or 10 bit digitization and still get 4.5 logs of linear dynamic
>range. A log conversion is done before the signal goes to the A/D
>convertor, so the 8 or 10 bit conversion does not decrease dynamic range.
>This is born out by the fact that many X-ray crystallographers (they need
>excellent signal to noise ratios) that use imaging plate systems prefer
>Fuji over the competition. (They also use their own software for data
>analysis, so Fuji's software isn't an issue for them).
>

Another thing 16 bits gives is a higher quality image.
    Tracy



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