Wizard DNA clean up
Jeffrey Shane Temple
jstemple at whale.st.usm.edu
Mon Dec 5 14:52:18 EST 1994
Richard Kenneth Bruce Martel (richardk at sfu.ca) wrote:
: I would appreciate a more elaborate protocol of how you purified your
: band from agarose. Was it high or low melt agarose? You said
: "binding buffer precipitated once, but washed away with column wash"
: what do you mean?
: The Wizard DNA clean up kit contains the resin, columns and a
: protocol, no buffers, washes etc. I talked to a tech at Promega and
: he said that the resin in each of their kits (ie. pcr preps, mini,
: midi and mega preps) are all different!!? Also, a flyer says that
: the DNA clean up kit can be used to gel purify DNA, the tech said
: that the PCR preps resin is better for gel purification.
: We have some older "Magic minipreps" laying around, as well as a new
: "midi prep" kit. I'm thinking of using the protocol in "Promega
: notes 43" and one of those resins to see if it will work.
: ps. If anyone from Promega is 'listening', I would welcome an
Same goes for me. Please send more details on your gel purification!!
Jeff Temple (jstemple at whale.st.usm.edu)
University of Southern MS
Dept. of Chem/Biochemistry
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