Converting recA to rec+

Michael Cooley szcooley at bullwinkle.ucdavis.edu
Mon Dec 5 12:21:37 EST 1994


Duncan Clark (Duncan at genesys.demon.co.uk) wrote:
: Hi folks,

: Does anyone have an easy way to change a recA1 E.coli (Invitrogen TOP10)
: back to rec+. I know I can do P1 transduction (assuming I can find
: something linked and selectable close to recA). Can I get revertants 
: of recA1 ie if I UV irradiate will I select for revertants etc?

: All help appreciated.

: Duncan   

: -----------------------------------------------------------------------------
: My mind's made up. Don't confuse me with the facts!


Why don't you simply transform with the recA gene. I know it has been cloned.


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